Povidone-iodine (betadine) in the treatment of experimental Pseudomonas aeruginosa keratitis

Kira Michalová, Andrew L. Moyes, Sarah Cameron, Billie Anne Juni, Wesley F. Obritsch, John A. Dvorak, Donald J. Doughman, Frank S. Rhame

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9 Scopus citations


Topical 5% povidone-iodine for the treatment of corneal ulcers was observed in Sierra Leone, West Africa by one of us (D.J.D.). To test the efficacy of topical 5% povidone-iodine for infectious keratitis, experimental Pseudomonas aeruginosa keratitis was induced in 12 rabbits by first abrading the central 3 mm of corneal epithelium. Thirty milliliters of broth of P. aeruginosa strain ATCC 27835 (1.8 x 107 viable bacteria) was dropped twice on the wounded cornea. After 22 h, all corneas were clinically infected. Eight rabbits were treated with 5% povidone-iodine solution and four with 0.9% NaCl solution. All were given hourly drops. Twenty-four hours after treatment began, the central 8-mm button of the infected cornea was excised, homogenized, and serial dilutions plated onto MacConkey agar. The total number of viable Pseudomonas organisms was calculated. The treatment group had 5.2±0.4 CFUs (colony-forming units) per cornea. The control group had 4.8±0.4 CFUs per cornea (p = 0.11). The clinical scores (Hobden grading system) were 6.9±1.5 for the treated group and 7.3±2.5 for the control group (p = 0.74). There was no statistical difference between the treated and control groups. Povidone-iodine (5%) is not effective in the acute treatment of P. aeruginosa keratitis in this rabbit model.

Original languageEnglish (US)
Pages (from-to)533-536
Number of pages4
Issue number5
StatePublished - Aug 27 1996


  • Keratitis
  • Povidone-iodine
  • Pseudomonas aeruginosa


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