Potential biomarkers of human salivary function: A modified proteomic approach

J. D. Rudney, R. K. Staikov, J. D. Johnson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Objective: In previous studies, we defined groups of subjects with opposite salivary function. Group membership was associated with clinically relevant outcomes. High aggregation-adherence (HAA) groups showed lower levels of caries, supragingival plaque, total streptococci, and Tannerella forsythensis than low high aggregation-adherence (LAA) groups. In this study, we used a proteomic approach to search for biomarkers which could be useful as risk indicators for those outcomes. Design: Clarified resting whole saliva from each of 41 HAA and LAA subjects was separated by preparative isoelectric focusing. Fractions showing the most distinctive protein profiles were pooled into four sets (pI 3-3.5, pI 4-4.7, pI 5.7-7.7, pI 10-11.5). Each pool then was compared by SDS-PAGE. Image analysis software was used to quantify matched bands. Partial least squares analysis (PLS) was used to determine which of the 65 bands from all four pools were the best predictors of group membership, caries, total plaque, total streptococci, and T. forsythensis counts. Those bands were identified by mass spectroscopy (MS-MS). Results: Two bands consistently were strong predictors in separate PLS analyses of each outcome variable. In follow-up univariate analyses, those bands showed the strongest significant differences between the HAA and LAA groups. They also showed significant inverse correlations with caries and all the microbiological variables. MSMS identified those bands as statherin, and a truncated cystatin S missing the first eight N-terminal amino acids. Conclusions: Levels of statherin and truncated cystatin S may be potential risk indicators for the development of caries and other oral diseases.

Original languageEnglish (US)
Pages (from-to)91-100
Number of pages10
JournalArchives of Oral Biology
Volume54
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Salivary Cystatins
Streptococcus
Least-Squares Analysis
Proteomics
Mouth Diseases
Biomarkers
Isoelectric Focusing
Saliva
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Software
Amino Acids
Proteins

Keywords

  • Biomarkers
  • Cystatin S
  • Dental biofilm
  • Dental caries
  • Proteomics
  • Salivary proteins
  • Statherin
  • Streptococci
  • Tannerella forsythensis

Cite this

Potential biomarkers of human salivary function : A modified proteomic approach. / Rudney, J. D.; Staikov, R. K.; Johnson, J. D.

In: Archives of Oral Biology, Vol. 54, No. 1, 01.01.2009, p. 91-100.

Research output: Contribution to journalArticle

Rudney, J. D. ; Staikov, R. K. ; Johnson, J. D. / Potential biomarkers of human salivary function : A modified proteomic approach. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 1. pp. 91-100.
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