Abstract
Objective: In previous studies, we defined groups of subjects with opposite salivary function. Group membership was associated with clinically relevant outcomes. High aggregation-adherence (HAA) groups showed lower levels of caries, supragingival plaque, total streptococci, and Tannerella forsythensis than low high aggregation-adherence (LAA) groups. In this study, we used a proteomic approach to search for biomarkers which could be useful as risk indicators for those outcomes. Design: Clarified resting whole saliva from each of 41 HAA and LAA subjects was separated by preparative isoelectric focusing. Fractions showing the most distinctive protein profiles were pooled into four sets (pI 3-3.5, pI 4-4.7, pI 5.7-7.7, pI 10-11.5). Each pool then was compared by SDS-PAGE. Image analysis software was used to quantify matched bands. Partial least squares analysis (PLS) was used to determine which of the 65 bands from all four pools were the best predictors of group membership, caries, total plaque, total streptococci, and T. forsythensis counts. Those bands were identified by mass spectroscopy (MS-MS). Results: Two bands consistently were strong predictors in separate PLS analyses of each outcome variable. In follow-up univariate analyses, those bands showed the strongest significant differences between the HAA and LAA groups. They also showed significant inverse correlations with caries and all the microbiological variables. MSMS identified those bands as statherin, and a truncated cystatin S missing the first eight N-terminal amino acids. Conclusions: Levels of statherin and truncated cystatin S may be potential risk indicators for the development of caries and other oral diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 91-100 |
| Number of pages | 10 |
| Journal | Archives of Oral Biology |
| Volume | 54 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2009 |
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Keywords
- Biomarkers
- Cystatin S
- Dental biofilm
- Dental caries
- Proteomics
- Salivary proteins
- Statherin
- Streptococci
- Tannerella forsythensis
Cite this
Potential biomarkers of human salivary function : A modified proteomic approach. / Rudney, J. D.; Staikov, R. K.; Johnson, J. D.
In: Archives of Oral Biology, Vol. 54, No. 1, 01.01.2009, p. 91-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potential biomarkers of human salivary function
T2 - A modified proteomic approach
AU - Rudney, J. D.
AU - Staikov, R. K.
AU - Johnson, J. D.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Objective: In previous studies, we defined groups of subjects with opposite salivary function. Group membership was associated with clinically relevant outcomes. High aggregation-adherence (HAA) groups showed lower levels of caries, supragingival plaque, total streptococci, and Tannerella forsythensis than low high aggregation-adherence (LAA) groups. In this study, we used a proteomic approach to search for biomarkers which could be useful as risk indicators for those outcomes. Design: Clarified resting whole saliva from each of 41 HAA and LAA subjects was separated by preparative isoelectric focusing. Fractions showing the most distinctive protein profiles were pooled into four sets (pI 3-3.5, pI 4-4.7, pI 5.7-7.7, pI 10-11.5). Each pool then was compared by SDS-PAGE. Image analysis software was used to quantify matched bands. Partial least squares analysis (PLS) was used to determine which of the 65 bands from all four pools were the best predictors of group membership, caries, total plaque, total streptococci, and T. forsythensis counts. Those bands were identified by mass spectroscopy (MS-MS). Results: Two bands consistently were strong predictors in separate PLS analyses of each outcome variable. In follow-up univariate analyses, those bands showed the strongest significant differences between the HAA and LAA groups. They also showed significant inverse correlations with caries and all the microbiological variables. MSMS identified those bands as statherin, and a truncated cystatin S missing the first eight N-terminal amino acids. Conclusions: Levels of statherin and truncated cystatin S may be potential risk indicators for the development of caries and other oral diseases.
AB - Objective: In previous studies, we defined groups of subjects with opposite salivary function. Group membership was associated with clinically relevant outcomes. High aggregation-adherence (HAA) groups showed lower levels of caries, supragingival plaque, total streptococci, and Tannerella forsythensis than low high aggregation-adherence (LAA) groups. In this study, we used a proteomic approach to search for biomarkers which could be useful as risk indicators for those outcomes. Design: Clarified resting whole saliva from each of 41 HAA and LAA subjects was separated by preparative isoelectric focusing. Fractions showing the most distinctive protein profiles were pooled into four sets (pI 3-3.5, pI 4-4.7, pI 5.7-7.7, pI 10-11.5). Each pool then was compared by SDS-PAGE. Image analysis software was used to quantify matched bands. Partial least squares analysis (PLS) was used to determine which of the 65 bands from all four pools were the best predictors of group membership, caries, total plaque, total streptococci, and T. forsythensis counts. Those bands were identified by mass spectroscopy (MS-MS). Results: Two bands consistently were strong predictors in separate PLS analyses of each outcome variable. In follow-up univariate analyses, those bands showed the strongest significant differences between the HAA and LAA groups. They also showed significant inverse correlations with caries and all the microbiological variables. MSMS identified those bands as statherin, and a truncated cystatin S missing the first eight N-terminal amino acids. Conclusions: Levels of statherin and truncated cystatin S may be potential risk indicators for the development of caries and other oral diseases.
KW - Biomarkers
KW - Cystatin S
KW - Dental biofilm
KW - Dental caries
KW - Proteomics
KW - Salivary proteins
KW - Statherin
KW - Streptococci
KW - Tannerella forsythensis
UR - http://www.scopus.com/inward/record.url?scp=56749177129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56749177129&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2008.08.007
DO - 10.1016/j.archoralbio.2008.08.007
M3 - Article
C2 - 18804197
AN - SCOPUS:56749177129
VL - 54
SP - 91
EP - 100
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 1
ER -