Stimulation or inhibition of hormone secretion from mammalian anterior pituitary cells in response to peptide releasing factors and neurotransmitters is accompanied by changes in membrane electrical activity and cellular calcium distribution. In addition, the levels of various intracellular second-messenger molecules (e. g. cyclic nucleotides and inoditol phospholipids) are modulated by secretogogue action in a manner which suggests that they serve to link receptor occupation to changes in electrical activity. Patch voltage clamp methods and TLC/HPLC measurements of inositol lipid metabolites have been applied to both neoplastic (GH3) and normal primary prolactin-secreting cells (lactotrophs) from rat in an effort to characterize and to determine the extent of involvement of specific ion channel types and inositol polyphosphates in such responses. The results are presented and discussed.
|Original language||English (US)|
|Title of host publication||IEEE/Engineering in Medicine and Biology Society Annual Conference|
|Number of pages||2|
|State||Published - Dec 1 1987|