TY - JOUR
T1 - Post-translational modifications of nuclear co-repressor RIP140
T2 - A therapeutic target for metabolic diseases
AU - Huq, M. D Mostaqul
AU - Gupta, Pawan
AU - Wei, Li-Na
PY - 2008/2
Y1 - 2008/2
N2 - Most proteins undergo post-translational modification (PTM), which is known to play roles in normal physiological processes and the progression of many diseases. In this review, we summarized and discussed the mass spectrometry (MS)-based studies of various PTMs of nuclear co-repressor, receptor interacting protein 140 (RIP140), as well as the significance of these PTMs in modulating the biological activities of RIP140, specifically in adipocytes. Comprehensive analyses of RIP140 by MS identified specific sites of PTMs on RIP140, including that of phosphorylation, acetylation, pyridoxylation, and protein arginine methylation. Studies of these PTMs revealed their combinatorial effects on the activities of RIP140 with respect to the regulation of hormone target genes and fat accumulation in adipocytes. These proteomic studies have presented evidence for the biological significanoe of specific PTMs of RIP140, and uncovered nutritional and physiological factors that trigger these PTMs in adipocytes. This could provide insights into potential, new therapeutic targets for diseases concerning adipocytes such as metabolic disorders.
AB - Most proteins undergo post-translational modification (PTM), which is known to play roles in normal physiological processes and the progression of many diseases. In this review, we summarized and discussed the mass spectrometry (MS)-based studies of various PTMs of nuclear co-repressor, receptor interacting protein 140 (RIP140), as well as the significance of these PTMs in modulating the biological activities of RIP140, specifically in adipocytes. Comprehensive analyses of RIP140 by MS identified specific sites of PTMs on RIP140, including that of phosphorylation, acetylation, pyridoxylation, and protein arginine methylation. Studies of these PTMs revealed their combinatorial effects on the activities of RIP140 with respect to the regulation of hormone target genes and fat accumulation in adipocytes. These proteomic studies have presented evidence for the biological significanoe of specific PTMs of RIP140, and uncovered nutritional and physiological factors that trigger these PTMs in adipocytes. This could provide insights into potential, new therapeutic targets for diseases concerning adipocytes such as metabolic disorders.
KW - Drug discovery
KW - Mass spectrometry
KW - Metabolic diseases
KW - Protein posttranslational modification
KW - Proteomics
KW - RIP140
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U2 - 10.2174/092986708783497382
DO - 10.2174/092986708783497382
M3 - Review article
C2 - 18288993
AN - SCOPUS:39449123776
SN - 0929-8673
VL - 15
SP - 386
EP - 392
JO - Current medicinal chemistry
JF - Current medicinal chemistry
IS - 4
ER -