Post-processing for statistical image analysis in light microscopy

Richard A. Cardullo, Edward H. Hinchcliffe

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Image processing of images serves a number of important functions including noise reduction, contrast enhancement, and feature extraction. Whatever the final goal, an understanding of the nature of image acquisition and digitization and subsequent mathematical manipulations of that digitized image is essential. Here we discuss the basic mathematical and statistical processes that are routinely used by microscopists to routinely produce high quality digital images and to extract key features of interest using a variety of extraction and thresholding tools.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages285-315
Number of pages31
DOIs
StatePublished - 2013

Publication series

NameMethods in Cell Biology
Volume114
ISSN (Print)0091-679X

Keywords

  • Contrast-enhancement
  • Convolution filters
  • Image processing
  • Lookup table
  • Noise reduction
  • Thresholding

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