Post implantation viability in canine allograft heart valves

Summary: Known viable allograft canine pulmonary valves were inserted in an isotopic position ensuring normal physiological function and simulating the clinical situation. Valves removed at periods up to eight weeks were assessed for viability using previously established criteria of tissue culture and protein and collagen synthesis. The origin of cells present within the allografted leaflets was investigated by chromosome identification where possible. The results, in association with the histological appearances, indicated that pre-implantation viability was not retained in the leaflets of the allografted valves implanted in this study.