TY - JOUR
T1 - Portable GMR Handheld Platform for the Detection of Influenza A Virus
AU - Wu, Kai
AU - Klein, Todd
AU - Krishna, Venkatramana D.
AU - Su, Diqing
AU - Perez, Andres M.
AU - Wang, Jian Ping
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/11/22
Y1 - 2017/11/22
N2 - Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.
AB - Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.
KW - Influenza A virus
KW - giant magnetoresistive
KW - nonclinical
KW - point-of-care device
KW - quantitative immunoassay platform
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U2 - 10.1021/acssensors.7b00432
DO - 10.1021/acssensors.7b00432
M3 - Article
C2 - 29068663
AN - SCOPUS:85034805956
SN - 2379-3694
VL - 2
SP - 1594
EP - 1601
JO - ACS Sensors
JF - ACS Sensors
IS - 11
ER -