Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.
Bibliographical noteFunding Information:
We thank Maxim Cheeran for his helpful suggestions. We acknowledge XPRIZE Foundation and Nokia Sensing XCHALLENGE competition for motivating the design of the Z-Lab Diagnosis Platform which won Distinguished Prize Award. This work is supported by MNDrive: OVPR STEMMA program, Institute of Engineering in Medicine of the University of Minnesota, National Science Foundation MRSEC facility program, the Distinguished McKnight University Professorship, Centennial Chair Professorship, Robert F Hartmann Endowed Chair, and UROP program from the University of Minnesota. Parts of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network (www.mrfn.org) via the MRSEC program.
© 2017 American Chemical Society.
Copyright 2018 Elsevier B.V., All rights reserved.
- Influenza A virus
- giant magnetoresistive
- point-of-care device
- quantitative immunoassay platform