Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance?

Apisit Kittawornrat, John Prickett, Wayne Chittick, Chong Wang, Mark Engle, Jeremy Johnson, Devi Patnayak, Trevor Schwartz, Daniel Whitney, Chris Olsen, Kent Schwartz, Jeffrey Zimmerman

Research output: Contribution to journalReview articlepeer-review

76 Scopus citations

Abstract

The purpose of this study was to determine whether oral fluid samples could be used to monitor individually-housed adult boars for porcine reproductive and respiratory syndrome virus (PRRSV) infection. In 3 trials, 24 boars were intramuscularly (IM) inoculated with a modified-live PRRSV (MLV) vaccine (Trial 1), a Type 1 PRRSV isolate (Trial 2), or a Type 2 isolate (Trial 3). Oral fluid samples were collected daily and serum samples were collected twice weekly. Following the completion of the study, samples were randomized and blind-tested for PRRSV by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). PRRSV was detected in oral fluids at DPI 1 and all oral fluid specimens were PRRSV qRT-PCR positive at DPI 4. Although PRRSV was detected in both serum and oral fluid specimens through DPI 21, a comparison of matched samples from individual boars showed that oral fluid was equal to serum for the detection of PRRSV at DPI 7 and more likely to be positive than serum on DPI 14 and 21. Overall, oral fluid was superior to serum for the detection of PRRSV using PCR over the 21-day observation period in this study. The results of this experiment suggest that individually-penned oral fluid sampling could be an efficient, cost-effective approach to PRRSV surveillance in boar studs and other swine populations.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalVirus research
Volume154
Issue number1-2
DOIs
StatePublished - Dec 2010

Bibliographical note

Funding Information:
This project was funded in part by the PRRS CAP USDA NIFA Award 2008-55620-19132 and received technical and product support from Life Technologies (Ricardo Munoz, DVM. Professional Services Veterinarian, Life Technologies, Austin, TX).

Keywords

  • Detection
  • Diagnosis
  • Monitor
  • Oral fluid
  • PRRSV
  • Serum
  • Surveillance

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