Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: Sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection

Steven R. Lawson; Kurt D. Rossow; James E. Collins; David A. Benfield; Raymond R R Rowland

doi:10.1016/S0168-1702(97)00086-5

Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: Sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection

Steven R. Lawson, Kurt D. Rossow, James E. Collins, David A. Benfield, Raymond R R Rowland

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.

Original language	English (US)
Pages (from-to)	105-113
Number of pages	9
Journal	Virus research
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/S0168-1702(97)00086-5
State	Published - Oct 1997

Bibliographical note

Funding Information:
We thank Jan Shirers for the MAC-387 immunohistochemistry. This work was supported by the USDA National Research Initiative for Competitive Grants Program Grant # 950224, National Science Foundation Grant # OSR-9108773, and by the South Dakota Future Fund.

Keywords

Gnotobiotic pigs
Porcine reproductive
Respiratory syndrome

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10.1016/S0168-1702(97)00086-5

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title = "Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: Sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection",

abstract = "The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.",

keywords = "Gnotobiotic pigs, Porcine reproductive, Respiratory syndrome",

author = "Lawson, {Steven R.} and Rossow, {Kurt D.} and Collins, {James E.} and Benfield, {David A.} and Rowland, {Raymond R R}",

note = "Funding Information: We thank Jan Shirers for the MAC-387 immunohistochemistry. This work was supported by the USDA National Research Initiative for Competitive Grants Program Grant # 950224, National Science Foundation Grant # OSR-9108773, and by the South Dakota Future Fund.",

year = "1997",

month = oct,

doi = "10.1016/S0168-1702(97)00086-5",

language = "English (US)",

volume = "51",

pages = "105--113",

journal = "Virus research",

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T1 - Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs

T2 - Sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection

AU - Lawson, Steven R.

AU - Rossow, Kurt D.

AU - Collins, James E.

AU - Benfield, David A.

AU - Rowland, Raymond R R

N1 - Funding Information: We thank Jan Shirers for the MAC-387 immunohistochemistry. This work was supported by the USDA National Research Initiative for Competitive Grants Program Grant # 950224, National Science Foundation Grant # OSR-9108773, and by the South Dakota Future Fund.

PY - 1997/10

Y1 - 1997/10

N2 - The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.

AB - The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.

KW - Gnotobiotic pigs

KW - Porcine reproductive

KW - Respiratory syndrome

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AN - SCOPUS:0031259987

SN - 0168-1702

VL - 51

SP - 105

EP - 113

JO - Virus research

JF - Virus research

IS - 2

ER -

Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: Sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection

Abstract

Bibliographical note

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