Porcine parvovirus: replication in and inhibition of selected cellular functions of swine alveolar macrophages and peripheral blood lymphocytes

M. J. Harding, T. W. Molitor

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The ability of four isolates of Porcine Parvovirus (NADL-8, NADL-2, KBSH, and Kresse) to replicate in and affect the functions of swine peripheral blood lymphocytes and alveolar macrophages was studied in vitro. V-strand and C-strand viral DNA was present in both concanavalin A- and non-treated lymphocytes as well as alveolar macrophages following infection with all four isolates. Indirect fluorescent antibody assays on swine testis cells, inoculated with cell lysates of NADL-8-infected peripheral blood lymphocytes (both concanavalin A- and non-treated) and alveolar macrophages, indicated that these immune cells supported the production of progeny virus. The quantity of viral DNA and progeny virus was dependent upon the multiplicity of infection and length of time following infection. Infection of lymphocytes and alveolar macrophages with PPV was associated with a decrease in cell viability. Peripheral blood mononuclear cells and alveolar macrophages infected with any of the four isolates demonstrated reduced lymphocyte blastogenesis and non-Fc-mediated alveolar macrophage phagocytosis, respectively.

Original languageEnglish (US)
Pages (from-to)105-117
Number of pages13
JournalArchives of Virology
Volume101
Issue number1-2
DOIs
StatePublished - Mar 1 1988

Fingerprint Dive into the research topics of 'Porcine parvovirus: replication in and inhibition of selected cellular functions of swine alveolar macrophages and peripheral blood lymphocytes'. Together they form a unique fingerprint.

Cite this