Pool of resistance mechanisms to glyphosate in digitaria insularis

Leonardo Bianco De Carvalho, Pedro Luis Da Costa Aguiar Alves, Fidel González-Torralva, Hugo Enrique Cruz-Hipolito, Antonia María Rojano-Delgado, Rafael De Prado, Javier Gil-Humanes, Francisco Barro, María Dolores Luque De Castro

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Digitaria insularis biotypes resistant to glyphosate have been detected in Brazil. Studies were carried out in controlled conditions to determine the role of absorption, translocation, metabolism, and gene mutation as mechanisms of glyphosate resistance in D. insularis. The susceptible biotype absorbed at least 12% more 14C-glyphosate up to 48 h after treatment (HAT) than resistant biotypes. High differential 14C-glyphosate translocation was observed at 12 HAT, so that >70% of the absorbed herbicide remained in the treated leaf in resistant biotypes, whereas 42% remained in the susceptible biotype at 96 HAT. Glyphosate was degraded to aminomethylphosphonic acid (AMPA), glyoxylate, and sarcosine by >90% in resistant biotypes, whereas a small amount of herbicide (up to 11%) was degraded by the susceptible biotype up to 168 HAT. Two amino acid changes were found at positions 182 and 310 in EPSPS, consisting of a proline to threonine and a tyrosine to cysteine substitution, respectively, in resistant biotypes. Therefore, absorption, translocation, metabolism, and gene mutation play an important role in the D. insularis glyphosate resistance.

Original languageEnglish (US)
Pages (from-to)615-622
Number of pages8
JournalJournal of agricultural and food chemistry
Volume60
Issue number2
DOIs
StatePublished - Jan 18 2012

Keywords

  • EPSPS
  • N-phosphonomethylglycine
  • mechanisms of resistance
  • sourgrass
  • weed resistance

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    De Carvalho, L. B., Alves, P. L. D. C. A., González-Torralva, F., Cruz-Hipolito, H. E., Rojano-Delgado, A. M., De Prado, R., Gil-Humanes, J., Barro, F., & Luque De Castro, M. D. (2012). Pool of resistance mechanisms to glyphosate in digitaria insularis. Journal of agricultural and food chemistry, 60(2), 615-622. https://doi.org/10.1021/jf204089d