TY - JOUR
T1 - Polyunsaturated fatty acids stimulate superoxide formation in tumor cells
T2 - A mechanism for specific cytotoxicity and a model for tumor necrosis factor?
AU - Peterson, Douglas A
AU - Mehta, N.
AU - Butterfield, J.
AU - Husak, M.
AU - Christopher, M. M.
AU - Jagarlapudi, S.
AU - Eaton, J. W.
PY - 1988/9/15
Y1 - 1988/9/15
N2 - Some neoplastic cell lines are readily killed when incubated in the presence of polyunsaturated fatty acids (PUFA). In an attempt to elucidate this phenomenon, we studied PUFA-driven superoxide (O2-) production by cultured NS-1 cells (murine lymphoid tumor cells). We find: (1) Even in the absence of added PUFA, NS-1 cells generate O2- (i.e., reduce nitroblue tetrazolium). (2) addition of PUFA increases O2- by >50%. (3) Artificial loading of NS-1 cells with liposome encapsulated superoxide dismutase prevents the majority of spontaneous and PUFA-driven NBT reduction. We conclude that PUFA drives O2- generation by tumor cells, that this generation is largely intracellular, and that this phenomenon may help explain toxicity of PUFA for tumor cells.
AB - Some neoplastic cell lines are readily killed when incubated in the presence of polyunsaturated fatty acids (PUFA). In an attempt to elucidate this phenomenon, we studied PUFA-driven superoxide (O2-) production by cultured NS-1 cells (murine lymphoid tumor cells). We find: (1) Even in the absence of added PUFA, NS-1 cells generate O2- (i.e., reduce nitroblue tetrazolium). (2) addition of PUFA increases O2- by >50%. (3) Artificial loading of NS-1 cells with liposome encapsulated superoxide dismutase prevents the majority of spontaneous and PUFA-driven NBT reduction. We conclude that PUFA drives O2- generation by tumor cells, that this generation is largely intracellular, and that this phenomenon may help explain toxicity of PUFA for tumor cells.
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U2 - 10.1016/S0006-291X(88)80600-4
DO - 10.1016/S0006-291X(88)80600-4
M3 - Article
C2 - 2844172
AN - SCOPUS:0023802836
SN - 0006-291X
VL - 155
SP - 1033
EP - 1037
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -