Polynitroxyl albumin inhibits inflammation and vasoocclusion in transgenic sickle mice

Hemchandra Mahaseth, Gregory M Vercellotti, Thomas E. Welch, Paul R. Bowlin, Khalid M. Sonbol, Carleton J.C. Hsia, Li Ma, John C Bischof, Robert P Hebbel, John D Belcher

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Individuals with sickle-cell disease (SCD) and transgenic sickle mice expressing human βS globin exhibit enhanced reactive oxygen species (ROS) production, vascular inflammation, and episodic vasoocclusion. We hypothesize that reduction of ROS will reduce endothelial-cell activation and adhesion-molecule expression, thereby inhibiting vasoocclusion. To test this hypothesis, we measured endothelial-cell activation, adhesion-molecule expression, and vasoocclusion in sickle mice after administering IV polynitroxyl albumin (PNA), a superoxide dismutase and catalase mimetic. Untreated sickle mice, compared with normal mice, showed increased activation of nuclear factor-κB (NF-κB), an oxidant-sensitive transcription factor, in their lungs, livers, and skin. NF-κB activation was increased further in the livers and skin of sickle but not normal mice after hypoxia-reoxygenation. IV administration of PNA inhibited NF-κB activation by 60% (P <. 01) in the lungs and by 33% (P <. 05) in the livers of sickle mice after hypoxia-reoxygenation. PNA also reduced the expression of vascular cell-adhesion molecule-1 (VCAM-1) by 57% in lung (P <. 05) and by 33% in liver (P <. 05) and reduced the expression of intercellular-adhesion molecule-1 (ICAM-1) by 40% in lung (P <. 05) and by 53% in liver (P <. 05). PNA inhibited a hypoxia-reoxygenation-induced increase in leukocyte rolling (P <. 01) and adhesion (P <. 05) in venules of the dorsal skin. Most importantly, PNA completely inhibited hypoxia-reoxygenation-induced vasoocclusion (P <. 001). Control albumin had no effect on NF-κB, VCAM-1, ICAM-1, rolling, adhesion, or vasoocclusion. We speculate that therapies to reduce oxidative stress will inhibit inflammation and vasoocclusion in SCD.

Original languageEnglish (US)
Pages (from-to)204-211
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume145
Issue number4
DOIs
StatePublished - Apr 1 2005

Fingerprint

Liver
Transgenic Mice
Chemical activation
Inflammation
Adhesion
Lung
Skin
Vascular Cell Adhesion Molecule-1
Endothelial cells
Cell Adhesion Molecules
Sickle Cell Anemia
Intercellular Adhesion Molecule-1
Reactive Oxygen Species
Endothelial Cells
Leukocyte Rolling
Molecules
Oxidative stress
Globins
Venules
Oxidants

Cite this

Polynitroxyl albumin inhibits inflammation and vasoocclusion in transgenic sickle mice. / Mahaseth, Hemchandra; Vercellotti, Gregory M; Welch, Thomas E.; Bowlin, Paul R.; Sonbol, Khalid M.; Hsia, Carleton J.C.; Ma, Li; Bischof, John C; Hebbel, Robert P; Belcher, John D.

In: Journal of Laboratory and Clinical Medicine, Vol. 145, No. 4, 01.04.2005, p. 204-211.

Research output: Contribution to journalArticle

Mahaseth, Hemchandra ; Vercellotti, Gregory M ; Welch, Thomas E. ; Bowlin, Paul R. ; Sonbol, Khalid M. ; Hsia, Carleton J.C. ; Ma, Li ; Bischof, John C ; Hebbel, Robert P ; Belcher, John D. / Polynitroxyl albumin inhibits inflammation and vasoocclusion in transgenic sickle mice. In: Journal of Laboratory and Clinical Medicine. 2005 ; Vol. 145, No. 4. pp. 204-211.
@article{8db76c58586e415788286154e6a56a4e,
title = "Polynitroxyl albumin inhibits inflammation and vasoocclusion in transgenic sickle mice",
abstract = "Individuals with sickle-cell disease (SCD) and transgenic sickle mice expressing human βS globin exhibit enhanced reactive oxygen species (ROS) production, vascular inflammation, and episodic vasoocclusion. We hypothesize that reduction of ROS will reduce endothelial-cell activation and adhesion-molecule expression, thereby inhibiting vasoocclusion. To test this hypothesis, we measured endothelial-cell activation, adhesion-molecule expression, and vasoocclusion in sickle mice after administering IV polynitroxyl albumin (PNA), a superoxide dismutase and catalase mimetic. Untreated sickle mice, compared with normal mice, showed increased activation of nuclear factor-κB (NF-κB), an oxidant-sensitive transcription factor, in their lungs, livers, and skin. NF-κB activation was increased further in the livers and skin of sickle but not normal mice after hypoxia-reoxygenation. IV administration of PNA inhibited NF-κB activation by 60{\%} (P <. 01) in the lungs and by 33{\%} (P <. 05) in the livers of sickle mice after hypoxia-reoxygenation. PNA also reduced the expression of vascular cell-adhesion molecule-1 (VCAM-1) by 57{\%} in lung (P <. 05) and by 33{\%} in liver (P <. 05) and reduced the expression of intercellular-adhesion molecule-1 (ICAM-1) by 40{\%} in lung (P <. 05) and by 53{\%} in liver (P <. 05). PNA inhibited a hypoxia-reoxygenation-induced increase in leukocyte rolling (P <. 01) and adhesion (P <. 05) in venules of the dorsal skin. Most importantly, PNA completely inhibited hypoxia-reoxygenation-induced vasoocclusion (P <. 001). Control albumin had no effect on NF-κB, VCAM-1, ICAM-1, rolling, adhesion, or vasoocclusion. We speculate that therapies to reduce oxidative stress will inhibit inflammation and vasoocclusion in SCD.",
author = "Hemchandra Mahaseth and Vercellotti, {Gregory M} and Welch, {Thomas E.} and Bowlin, {Paul R.} and Sonbol, {Khalid M.} and Hsia, {Carleton J.C.} and Li Ma and Bischof, {John C} and Hebbel, {Robert P} and Belcher, {John D}",
year = "2005",
month = "4",
day = "1",
doi = "10.1016/j.lab.2005.02.008",
language = "English (US)",
volume = "145",
pages = "204--211",
journal = "Translational research : the journal of laboratory and clinical medicine",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Polynitroxyl albumin inhibits inflammation and vasoocclusion in transgenic sickle mice

AU - Mahaseth, Hemchandra

AU - Vercellotti, Gregory M

AU - Welch, Thomas E.

AU - Bowlin, Paul R.

AU - Sonbol, Khalid M.

AU - Hsia, Carleton J.C.

AU - Ma, Li

AU - Bischof, John C

AU - Hebbel, Robert P

AU - Belcher, John D

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Individuals with sickle-cell disease (SCD) and transgenic sickle mice expressing human βS globin exhibit enhanced reactive oxygen species (ROS) production, vascular inflammation, and episodic vasoocclusion. We hypothesize that reduction of ROS will reduce endothelial-cell activation and adhesion-molecule expression, thereby inhibiting vasoocclusion. To test this hypothesis, we measured endothelial-cell activation, adhesion-molecule expression, and vasoocclusion in sickle mice after administering IV polynitroxyl albumin (PNA), a superoxide dismutase and catalase mimetic. Untreated sickle mice, compared with normal mice, showed increased activation of nuclear factor-κB (NF-κB), an oxidant-sensitive transcription factor, in their lungs, livers, and skin. NF-κB activation was increased further in the livers and skin of sickle but not normal mice after hypoxia-reoxygenation. IV administration of PNA inhibited NF-κB activation by 60% (P <. 01) in the lungs and by 33% (P <. 05) in the livers of sickle mice after hypoxia-reoxygenation. PNA also reduced the expression of vascular cell-adhesion molecule-1 (VCAM-1) by 57% in lung (P <. 05) and by 33% in liver (P <. 05) and reduced the expression of intercellular-adhesion molecule-1 (ICAM-1) by 40% in lung (P <. 05) and by 53% in liver (P <. 05). PNA inhibited a hypoxia-reoxygenation-induced increase in leukocyte rolling (P <. 01) and adhesion (P <. 05) in venules of the dorsal skin. Most importantly, PNA completely inhibited hypoxia-reoxygenation-induced vasoocclusion (P <. 001). Control albumin had no effect on NF-κB, VCAM-1, ICAM-1, rolling, adhesion, or vasoocclusion. We speculate that therapies to reduce oxidative stress will inhibit inflammation and vasoocclusion in SCD.

AB - Individuals with sickle-cell disease (SCD) and transgenic sickle mice expressing human βS globin exhibit enhanced reactive oxygen species (ROS) production, vascular inflammation, and episodic vasoocclusion. We hypothesize that reduction of ROS will reduce endothelial-cell activation and adhesion-molecule expression, thereby inhibiting vasoocclusion. To test this hypothesis, we measured endothelial-cell activation, adhesion-molecule expression, and vasoocclusion in sickle mice after administering IV polynitroxyl albumin (PNA), a superoxide dismutase and catalase mimetic. Untreated sickle mice, compared with normal mice, showed increased activation of nuclear factor-κB (NF-κB), an oxidant-sensitive transcription factor, in their lungs, livers, and skin. NF-κB activation was increased further in the livers and skin of sickle but not normal mice after hypoxia-reoxygenation. IV administration of PNA inhibited NF-κB activation by 60% (P <. 01) in the lungs and by 33% (P <. 05) in the livers of sickle mice after hypoxia-reoxygenation. PNA also reduced the expression of vascular cell-adhesion molecule-1 (VCAM-1) by 57% in lung (P <. 05) and by 33% in liver (P <. 05) and reduced the expression of intercellular-adhesion molecule-1 (ICAM-1) by 40% in lung (P <. 05) and by 53% in liver (P <. 05). PNA inhibited a hypoxia-reoxygenation-induced increase in leukocyte rolling (P <. 01) and adhesion (P <. 05) in venules of the dorsal skin. Most importantly, PNA completely inhibited hypoxia-reoxygenation-induced vasoocclusion (P <. 001). Control albumin had no effect on NF-κB, VCAM-1, ICAM-1, rolling, adhesion, or vasoocclusion. We speculate that therapies to reduce oxidative stress will inhibit inflammation and vasoocclusion in SCD.

UR - http://www.scopus.com/inward/record.url?scp=21044448516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21044448516&partnerID=8YFLogxK

U2 - 10.1016/j.lab.2005.02.008

DO - 10.1016/j.lab.2005.02.008

M3 - Article

VL - 145

SP - 204

EP - 211

JO - Translational research : the journal of laboratory and clinical medicine

JF - Translational research : the journal of laboratory and clinical medicine

SN - 1931-5244

IS - 4

ER -