Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

E. Castellanos, A. Aranaz, B. Romero, L. De Juan, J. Alvarez, J. Bezos, S. Rodríguez, K. Stevenson, A. Mateos, L. Domínguez

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

Original languageEnglish (US)
Pages (from-to)3439-3442
Number of pages4
JournalJournal of clinical microbiology
Volume45
Issue number10
DOIs
StatePublished - Oct 2007

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

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