Polymeric proteins and their association with grain yield in hard red spring wheat lines

Toi J. Tsilo, Gary A. Hareland, Jae Bom Ohm, James A. Anderson

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Development of high yielding wheat (Triticum aestivum L.) varieties with acceptable end-use quality is a major focus in breeding programs worldwide. Variations in molecular weight (Mw) distribution of endosperm proteins are known to influence end-use quality traits. This paper reports the relationship of the size-exclusion high performance liquid chromatography (SE-HPLC) profile of endosperm proteins with grain yield. Flour samples were previously analyzed for Mw distribution of sodium-dodecyl sulfate (SDS) extractable and unextractable proteins using the SE-HPLC protocol. Correlations were calculated between grain yield and HPLC absorbance data obtained at 0.01-min retention time intervals. Although both SDS-extractable and unextractable proteins had positive correlations with grain protein content, only SDS-unextractable very high Mw polymeric proteins (UVHP) had no negative association with grain yield, while SDS-extractable fractions rich in low Mw polymeric proteins had a negative correlation (r = -0.41) with grain yield. These results suggest that in an effort to increase grain yield, breeding programs should target grain yield and also increase levels of UVHP and decrease SDS-extractable polymeric proteins, thereby, maintaining acceptable bread-making quality.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
Issue number2
StatePublished - Nov 2013

Bibliographical note

Funding Information:
Acknowledgments The authors gratefully acknowledge financial support from the Minnesota Annual Conference of the United Methodist Church, the Compton International Foundation, the National Research Foundation of South Africa, the Agricultural Research Council of South Africa, and the National Research Initiative of USDA’s Cooperative State Research, Education, and Extension Service, CAP Grant No. 2006-55606-16629. The work performed at Hard Spring and Durum Wheat Quality Laboratory USDA-ARS was supported by CRIS Project No. 5442-43440-008-00D.


  • Grain protein content
  • Grain yield
  • SDS-extractable polymeric proteins
  • SDS-unextractable polymeric proteins
  • Wheat bread-making quality


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