Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation: Nanoparticles antidegradation activity at bonded dentin

Manuel Toledano-Osorio, Raquel Osorio, Fátima S. Aguilera, Antonio Luis Medina-Castillo, Manuel Toledano, Estrella Osorio, Sergio Acosta, Ruoqiong Chen, Conrado Aparicio

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-Flow Reactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation but stored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanical assessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysis were performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51% for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteria reduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, the highest complex modulus values at the resin-dentin interface over time. Regarding the mineral content, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associated to the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPs application permitted to observe remineralization of the etched and non-resin infiltrated collagen layer, and bacteria were scarcely encountered. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exerted an antibacterial role but did not remineralize the bonded interface. Undoped-NPs did not improve the properties of the interfaces. Application of Zn-doped NPs during the bonding procedure is encouraged. Statement of significance: Application of Zn-doped nanoparticles on acid etched dentin reduced biofilm formation and viability at the resin-dentin interface due to both remineralization and antibacterial properties. Doxycycline-doped nanoparticles also diminished oral biofilm viability, but did not remineralize the resin-dentin interface.

Original languageEnglish (US)
Pages (from-to)316-326
Number of pages11
JournalActa Biomaterialia
Volume111
DOIs
StatePublished - Jul 15 2020

Bibliographical note

Funding Information:
This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers ( http://uic.umn.edu) .

Funding Information:
This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE] and University of Granada Research & Transfer Program. The authors acknowledge Professor Joel D. Rudney, University of Minnesota for facilitating the use of microbiology facilities and access to the plaque samples. Multiphoton confocal laser microscope was performed at the University of Minnesota Imaging Centers (http://uic.umn.edu). The authors report no conflicts of interest in this work.

Publisher Copyright:
© 2020

Keywords

  • Biofilm
  • Degradation
  • Dentin
  • Interface
  • Nanoparticle

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