Reação em cadeia da polimerase para o diagnóstico de campilobacteriose genital bovina

Translated title of the contribution: Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

Ana C.M. Groff, Jackeline K. Kirinus, Mariana Sá e Silva, Gustavo Machado, Mateus M. Costa, Agueda P.C. Vargas

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Bovine genital campylobacteriosis is a common venereal disease of cattle; the prevalence of this disease can be underestimated mostly because of the nature of the etiological agent, the microaerobic Campylobacter fetus subspecies venerealis. The purpose of the current study was to evaluate the utilization of polymerase chain reaction (PCR) in the diagnosis of genital campylobacteriosis in samples obtained from bull prepuce aspirate, cow cervical mucus, and abomasum contents of aborted fetuses, collected into enrichment medium. Five different DNA extraction protocols were tested: thermal extraction, lysis with proteinase K, lysis with guanidine isothiocyanate, lysis with DNAzol, and lysis with hexadecyltrimethylammonium bromide (CTAB). The specificity, sensitivity, and technical application of the PCR assay were also evaluated with clinical samples and compared to bacterial isolation by standard culture. DNA extraction by the CTAB protocol provided better results in PCR, and it was able to detect 63 colony-forming units per ml of C. fetus. Out of 277 clinical samples tested, 68 (24%) were positive for Campylobacter fetus using PCR, while only 8 (2.8%) of the samples were positive by bacterial isolation in solid medium, proving the superiority of the PCR technique when compared to the standard isolation method, and providing evidence for its usefulness as a better screening test in cattle for the diagnosis of bovine genital campylobacteriosis.

Translated title of the contributionPolymerase chain reaction for the diagnosis of bovine genital campylobacteriosis
Original languagePortuguese
Pages (from-to)1031-1035
Number of pages5
JournalPesquisa Veterinaria Brasileira
Volume30
Issue number12
DOIs
StatePublished - 2010

Keywords

  • Abortion
  • Bovine genital campylobacteriosis
  • Campylobacter fetus
  • Diagnosis

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