Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex-Wide Opto-Electrophysiological Recordings

Preston D. Donaldson, Zahra S. Navabi, Russell E. Carter, Skylar M.L. Fausner, Leila Ghanbari, Timothy J. Ebner, Sarah L. Swisher, Suhasa B. Kodandaramaiah

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Electrophysiology and optical imaging provide complementary neural sensing capabilities – electrophysiological recordings have high temporal resolution, while optical imaging allows recording of genetically-defined populations at high spatial resolution. Combining these two modalities for simultaneous large-scale, multimodal sensing of neural activity across multiple brain regions can be very powerful. Here, transparent, inkjet-printed electrode arrays with outstanding optical and electrical properties are seamlessly integrated with morphologically conformant transparent polymer skulls. Implanted on transgenic mice expressing the Calcium (Ca2+) indicator GCaMP6f in excitatory neurons, these “eSee-Shells” provide a robust opto-electrophysiological interface for over 100 days. eSee-Shells enable simultaneous mesoscale Ca2+ imaging and electrocorticography (ECoG) acquisition from multiple brain regions covering 45 mm2 of cortex under anesthesia and in awake animals. The clarity and transparency of eSee-Shells allow recording single-cell Ca2+ signals directly below the electrodes and interconnects. Simultaneous multimodal measurement of cortical dynamics reveals changes in both ECoG and Ca2+ signals that depend on the behavioral state.

Original languageEnglish (US)
Article number2200626
JournalAdvanced Healthcare Materials
Volume11
Issue number18
DOIs
StatePublished - Sep 21 2022

Bibliographical note

Funding Information:
S.B.K., S.L.S., and T.J.E. acknowledge the NINDS Award #R0NS111028. S.B.K. acknowledges the Brain Initiative Award R42NS110165. Microfabrication and PSF characterizations were performed at the Minnesota Nano Center, funded by NSF NNCI Award ECCS-2025124. P.D.D. was supported by NSF IGERT Award DGE-1069104. S.B.K. and T.J.E. acknowledge P30DA048742.

Funding Information:
S.B.K., S.L.S., and T.J.E. acknowledge the NINDS Award #R0NS111028. S.B.K. acknowledges the Brain Initiative Award R42NS110165. Microfabrication and PSF characterizations were performed at the Minnesota Nano Center, funded by NSF NNCI Award ECCS‐2025124. P.D.D. was supported by NSF IGERT Award DGE‐1069104. S.B.K. and T.J.E. acknowledge P30DA048742.

Publisher Copyright:
© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

Keywords

  • calcium imaging
  • cortex-wide recording
  • electrophysiology
  • multi-modal recording
  • transparent electrodes

PubMed: MeSH publication types

  • Journal Article

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