Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) hold great potential in many areas of research such as cardiac tissue regeneration, cardiotoxicity screening and human heart disease models; however, before any of these applications can be realized, hESC-CMs need to progress from an immature phenotype to one that more closely resembles their adult counterparts in vivo. Current immature hESC-CMs can be characterized by their rounded morphology, disorganized contractile apparatus and circumferential gap junction expression. In an effort to improve the maturation of hESCCMs, prior work in our lab used micropatterned lanes of Matrigel and fibronectin extracellular matrix proteins on glass slides to control cell shape. From these experiments it was found that widths ranging from 30 to 80 μm promoted the best sarcomere development and nuclear alignment in a pure population of hESC-CMs. In this new system, a pure population of hESCCMs are seeded onto lanes of Matrigel on polydimethylsiloxane (PDMS) to investigate the portability of this technique to other substrate systems and how the substrate stimuli influences maturation. The same trend in nuclear alignment and sarcomere organization with lane width was observed when hESC-CMs were seeded onto lanes of Matrigel on PDMS as was found in the experiments utilizing glass as the substrate. By restricting cell adhesion and controlling substrate stiffness it may be possible to enhance the maturation of hESC-CMs in vitro which will provide a more physiological relevant phenotype that can then be used in the aforementioned applications.
|Original language||English (US)|
|Title of host publication||Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics|
|Editors||Chad S. Korach, Srinivasan Arjun Tekalur, Pablo Zavattieri|
|Publisher||Springer New York LLC|
|Number of pages||7|
|State||Published - 2016|
|Event||SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2015 - Costa Mesa, United States|
Duration: Jun 8 2015 → Jun 11 2015
|Name||Conference Proceedings of the Society for Experimental Mechanics Series|
|Conference||SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2015|
|Period||6/8/15 → 6/11/15|
Bibliographical noteFunding Information:
This research was supported with funds from the National Institutes of Health Grant K18 HL105504 from the Heart, Blood and Lung Institute and the Graduate School of the University of Wisconsin-Madison.
© The Society for Experimental Mechanics, Inc. 2016.
Copyright 2016 Elsevier B.V., All rights reserved.
- Cardiac tissue engineering
- Microcontact printing
- Stem cell