Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy

Jie Liu, Youxi Zhao, Mengjie Diao, Wanqing Wang, Wei Hua, Shuang Wu, Paul L Chen, R. R Ruan, Yanling Cheng

Research output: Contribution to journalArticle

Abstract

Polyhydroxyalkanoates (PHAs) are microbially synthesized biopolyesters which have attracted great attentions as a new biological material, potential alternative to traditional fossil fuel-based plastic due to their biodegradability and biocompatibility. Poly-3-hydroxybutyrate (PHB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are the most common members of PHAs. In this study, the nonsulfur and facultatively phototrophic bacterium Rhodospirillum rubrum was cultivated to accumulate PHA by a two-step culture strategy. Gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance spectroscopy (NMR) analyses showed that PHAs synthesized from fructose was PHBV, in which the 3HV content was 46.5 mol%, which means the better mechanical property. The molecular weight, distribution, and thermal features were characterized by gel permeation chromatography (GPC), differential scanning calorimeter (DSC), and thermo gravimetric analysis (TGA), respectively. The low PDI of 1.08 revealed the narrow and evenly molar mass distribution which shows the stable features. The high melting temperature and their other physical properties implied their potential applications. The traditional process of producing PHBV involves related carbon sources such as valeric acid. However, our study clearly described a new medium formula with fructose and a complete fermentation method to produce PHBV with a high 3HV faction and low molecular distribution.

Original languageEnglish (US)
Article number8369179
JournalJournal of Chemistry
Volume2019
DOIs
StatePublished - Jan 1 2019

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Polyhydroxyalkanoates
Fructose
Gas chromatography
Molar mass
Biodegradability
Gel permeation chromatography
Molecular weight distribution
Calorimeters
Biocompatibility
Fossil fuels
Biological materials
Fermentation
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Melting point
Thermogravimetric analysis
Bacteria
Carbon
Physical properties
Plastics

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy. / Liu, Jie; Zhao, Youxi; Diao, Mengjie; Wang, Wanqing; Hua, Wei; Wu, Shuang; Chen, Paul L; Ruan, R. R; Cheng, Yanling.

In: Journal of Chemistry, Vol. 2019, 8369179, 01.01.2019.

Research output: Contribution to journalArticle

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