Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

B. Swarnalatha, S. L. Nair, K. T. Shalumon, L. C. Milbauer, R. Jayakumar, B. Paul-Prasanth, K. K. Menon, R. P. Hebbel, A. Somani, Shantikumar V. Nair

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume58
DOIs
StatePublished - Jul 2013

Bibliographical note

Funding Information:
The authors are thankful to the Indian Council for Medical Research (ICMR) , Government of India as well as to the University of Minnesota (UMN) for grant funding (ICMR-UMN Joint Initiative, Ref. ID: 5/3/8/3/UOM/2011-RHN). The authors are grateful to Amrita Vishwa Vidyapeetham for providing the infrastructural support for this work.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • BOEC
  • Chitosan
  • Collagen
  • ENOS
  • Nanofibers
  • Poly (lactic acid)
  • Wound healing

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