Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

B. Swarnalatha, S. L. Nair, K. T. Shalumon, Liming Milbauer, R. Jayakumar, B. Paul-Prasanth, K. K. Menon, Robert P Hebbel, Arif Somani, Shantikumar V. Nair

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume58
DOIs
StatePublished - Jul 1 2013

Fingerprint

Nanofibers
Endothelial cells
Chitosan
Rats
Blood
Collagen
Endothelial Cells
Scaffolds
Nitric Oxide Synthase Type III
Composite materials
Substrates
Wound Healing
poly(lactic acid)

Keywords

  • BOEC
  • Chitosan
  • Collagen
  • ENOS
  • Nanofibers
  • Poly (lactic acid)
  • Wound healing

Cite this

Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells. / Swarnalatha, B.; Nair, S. L.; Shalumon, K. T.; Milbauer, Liming; Jayakumar, R.; Paul-Prasanth, B.; Menon, K. K.; Hebbel, Robert P; Somani, Arif; Nair, Shantikumar V.

In: International Journal of Biological Macromolecules, Vol. 58, 01.07.2013, p. 220-224.

Research output: Contribution to journalArticle

Swarnalatha, B. ; Nair, S. L. ; Shalumon, K. T. ; Milbauer, Liming ; Jayakumar, R. ; Paul-Prasanth, B. ; Menon, K. K. ; Hebbel, Robert P ; Somani, Arif ; Nair, Shantikumar V. / Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells. In: International Journal of Biological Macromolecules. 2013 ; Vol. 58. pp. 220-224.
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AU - Jayakumar, R.

AU - Paul-Prasanth, B.

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