Polarized distribution of Na+, K+-ATPase in giant cells elicited in vivo and in vitro

A. Vignery, T. Niven-Fairchild, D. H. Ingbar, M. Caplan

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28 Scopus citations


Giant cell formation was analyzed to determine whether it results in the high level of Na+, K+-ATPase expression that characterizes multinucleated cells such as osteoclasts. Giant cells and fusing alveolar macrophages were subjected to morphological, immunological, and biochemical studies. Both subunits of the Na+, K+-ATPase were found to be present on the plasma membrane of giant cells. Their localization was restricted to the non-adherent domain of the cell surface. Dynamic studies of giant cell differentiation demonstrated that on culture and/or multinucleation, an increase in sodium pump α-subunit synthesis occurred and led to a high level of expression of Na pumps. Conversely, the adherent plasma membrane of giant cells was enriched in a lysosomal membrane antigen. This study demonstrates that culture and/or multinucleation induces a significant increase in the expression of sodium pumps. The polarized distribution of these pumps and of a lysosomal component suggests that fusing macrophages undergo biochemical and morphological alterations which prepare them for a new and specialized function in chronic inflammatory reactions. Giant cells may offer a suitable model system to study the differentiation of other related multinucleated cells, such as osteoclasts.

Original languageEnglish (US)
Pages (from-to)1265-1271
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Issue number8
StatePublished - 1989


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