Polarization-sensitive optical coherence tomography reveals gray matter and white matter atrophy in SCA1 mouse models

Chao J. Liu, Orion Rainwater, H. Brent Clark, Harry T Orr, Taner Akkin

Research output: Contribution to journalArticle

Abstract

Spinocerebellar ataxia type 1 (SCA1) is a fatal inherited neurodegenerative disease. In this study, we demonstrate the label-free optical imaging methodology that can detect, with a high degree of sensitivity, discrete areas of degeneration in the cerebellum of the SCA1 mouse models. We used ATXN1[82Q] and ATXN1[30Q]-D776 mice in which the transgene is directed only to Purkinje cells. Molecular layer, granular layer, and white matter regions are analyzed using the intrinsic contrasts provided by polarization-sensitive optical coherence tomography. Cerebellar atrophy in SCA1 mice occurred both in gray matter and white matter. While gray matter atrophy is obvious, indications of white matter atrophy including different birefringence characteristics, and shortened and contorted branches are observed. Imaging results clearly show the loss or atrophy of myelinated axons in ATXN1[82Q] mice. The method provides unbiased contrasts that can facilitate the understanding of the pathological progression in neurodegenerative diseases and other neural disorders.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalNeurobiology of Disease
Volume116
DOIs
StatePublished - Aug 1 2018

Fingerprint

Spinocerebellar Ataxias
Optical Coherence Tomography
Atrophy
Neurodegenerative Diseases
Birefringence
Purkinje Cells
Optical Imaging
Transgenes
Cerebellum
Axons
White Matter
Gray Matter

Keywords

  • Ataxia
  • Neurodegeneration
  • Optical coherence tomography
  • Polarization

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Polarization-sensitive optical coherence tomography reveals gray matter and white matter atrophy in SCA1 mouse models. / Liu, Chao J.; Rainwater, Orion; Clark, H. Brent; Orr, Harry T; Akkin, Taner.

In: Neurobiology of Disease, Vol. 116, 01.08.2018, p. 69-77.

Research output: Contribution to journalArticle

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