Pneumococcal peptidoglycan-polysaccharides induce the expression of interleukin-8 in airway epithelial cells by way of nuclear factor-κB, nuclear factor interleukin-6, or activation protein-1 dependent mechanisms

Katsuyuki Tsuchiya, Katsuhiro Toyama, Vladimir Tsuprun, Yuki Hamajima, Youngki Kim, Frank G Ondrey, Jizhen Lin

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Cell envelope compounds of bacteria trigger immune and inflammatory reactions by way of chemokines/cytokines. In this study, we demonstrated that pneumococcal peptidoglycan-polysaccharides (PGPS) induced the production of interleukin (IL)-8 by way of nuclear factor (NF)-κB, nuclear factor interleukin (NF-IL)6, and activation protein (AP)-1 dependent mechanisms in the human bronchial epithelial cells (NL-20) in a dose- and time-dependent manner in vitro, and the mutation of either the NF-κB, NF-IL6, or AP-1 binding sites in the promoter of IL-8 abrogated the IL-8 transcriptional activity. In a similar way, lipopolysaccharides induced the promoter activation of IL-8 in NL-20. However, the PGPS-induced IL-8 promoter activation in rodent middle ear epithelial cells required NF-κB and NF-IL6 but not AP-1.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalLaryngoscope
Volume117
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Bronchial epithelial cell
  • Human
  • Interleukin-8
  • Middle ear epithelial cell
  • Peptidoglycan-polysaccharides
  • Rodent

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