Abstract
Cell envelope compounds of bacteria trigger immune and inflammatory reactions by way of chemokines/cytokines. In this study, we demonstrated that pneumococcal peptidoglycan-polysaccharides (PGPS) induced the production of interleukin (IL)-8 by way of nuclear factor (NF)-κB, nuclear factor interleukin (NF-IL)6, and activation protein (AP)-1 dependent mechanisms in the human bronchial epithelial cells (NL-20) in a dose- and time-dependent manner in vitro, and the mutation of either the NF-κB, NF-IL6, or AP-1 binding sites in the promoter of IL-8 abrogated the IL-8 transcriptional activity. In a similar way, lipopolysaccharides induced the promoter activation of IL-8 in NL-20. However, the PGPS-induced IL-8 promoter activation in rodent middle ear epithelial cells required NF-κB and NF-IL6 but not AP-1.
Original language | English (US) |
---|---|
Pages (from-to) | 86-91 |
Number of pages | 6 |
Journal | Laryngoscope |
Volume | 117 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2007 |
Keywords
- Bronchial epithelial cell
- Human
- Interleukin-8
- Middle ear epithelial cell
- Peptidoglycan-polysaccharides
- Rodent