Ploidy reduction in Saccharomyces cerevisiae

Aleeza Gerstein, Rachel M. McBride, Sarah P. Otto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Large-scale transitions in genome size from tetraploid to diploid were observed during a previous 1800-generation evolution experiment in Saccharomyces cerevisiae. Whether the transitions occurred via a one-step process (tetraploid to diploid) or through multiple steps (through ploidy intermediates) remained unclear. To provide insight into the mechanism involved, we investigated whether triploid-sized cells sampled from the previous experiment could also undergo ploidy loss. A batch culture experiment was conducted for approximately 200 generations, starting from four triploid-sized colonies and one contemporaneous tetraploid-sized colony. Ploidy reduction towards diploidy was observed in both triploid and tetraploid lines. Comparative genomic hybridization indicated the presence of aneuploidy in both the founder and the evolved colonies. The specific aneuploidies involved suggest that chromosome loss was not haphazard but that nearly full sets of chromosomes were lost at once, with some additional chromosome mis-segregation events. These results suggest the existence of a mitotic mechanism allowing the elimination of an entire set of chromosomes in S. cerevisiae, thereby reducing the ploidy level.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
JournalBiology Letters
Volume4
Issue number1
DOIs
StatePublished - Feb 23 2008

Fingerprint

Tetraploidy
Ploidies
ploidy
Triploidy
tetraploidy
Saccharomyces cerevisiae
triploidy
Diploidy
diploidy
Chromosomes
aneuploidy
Aneuploidy
chromosomes
comparative genomic hybridization
chromosome elimination
Genome Size
Chromosome Segregation
Batch Cell Culture Techniques
Comparative Genomic Hybridization
genome

Keywords

  • Aneuploidy
  • Ploidy
  • Yeast

Cite this

Gerstein, A., McBride, R. M., & Otto, S. P. (2008). Ploidy reduction in Saccharomyces cerevisiae. Biology Letters, 4(1), 91-94. https://doi.org/10.1098/rsbl.2007.0476

Ploidy reduction in Saccharomyces cerevisiae. / Gerstein, Aleeza; McBride, Rachel M.; Otto, Sarah P.

In: Biology Letters, Vol. 4, No. 1, 23.02.2008, p. 91-94.

Research output: Contribution to journalArticle

Gerstein, A, McBride, RM & Otto, SP 2008, 'Ploidy reduction in Saccharomyces cerevisiae', Biology Letters, vol. 4, no. 1, pp. 91-94. https://doi.org/10.1098/rsbl.2007.0476
Gerstein, Aleeza ; McBride, Rachel M. ; Otto, Sarah P. / Ploidy reduction in Saccharomyces cerevisiae. In: Biology Letters. 2008 ; Vol. 4, No. 1. pp. 91-94.
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