Pleiotropic changes in cycloheximide-resistant insect cell clones

Nassim Nouri, Ann Marie Fallon

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Somatic cell mutants resistant to drugs that interact with the eukaryotic ribosome provide a useful tool for studies on ribosome structure, function, and genetics. From Aedes albopictus (mosquito) cells, cycloheximide-resistant mutants (Cx-705 and Cx-738) that were about 30-fold more resistant to cycloheximide than the parental cells have been obtained. The observation that protein synthesis in cell-free lysates from Cx-705 and Cx-738 cells was resistant to cycloheximide led us to suspect that the alteration in these mutants might affect the ribosome. The present studies show that the cycloheximide-resistant cells grow poorly and eventually die at 34.5°C, a temperature at which wild-type cells grow normally. Relative to control cells, the cycloheximide-resistant cells show there were no differences between cycloheximide-resistant cells and wild-type cells in sensitivity to puromycin, emetine, or cryptopleurine. Cx-705 cells were predominantly diploid; in contrast, the frequency of tetraploid nuclei in Cx-738 cells was about 40%.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Issue number3
StatePublished - Mar 1987


  • Ardes albopictus
  • cycloheximide
  • mosquito
  • ribosome


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