Platelet-derived growth factor blocks the increase in intracellular free Ca2+ caused by calcium ionophores and a volatile anesthetic agent in Swiss 3T3 fibroblasts without altering toxicity

R. A. Olsen, M. J. Seewald, D. C. Melder, M. Berggren, P. A. Iaizzo, G. Powis

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10 Scopus citations

Abstract

Platelet-derived growth factor (PDGF) produced an almost complete block of the increase in intracellular free Ca2+ concentration ([Ca2+]i) in Swiss 3T3 fibroblasts caused by the Ca2+-selective ionophores 4-bromo-A23187 and ionomycin, and by the volatile anesthetic agent halothane. The effect of PDGF was similar to the decreased [Ca2+]i response to Ca2+-ionophores produced by phorbol 12-myristate 13-acetate, an activator of protein kinase C. There was no effect of PDGF or PMA on the acute or delayed toxicity of the Ca2+-ionophores to Swiss 3T3 cells, suggesting that the increase in [Ca2+]i, is not the direct cause of toxicity of these agents.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalToxicology Letters
Volume55
Issue number1
DOIs
StatePublished - Jan 1991

Keywords

  • Ca Ionophores
  • Halothane
  • PDGF
  • Swiss 3T3 cells

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