Plasticity in the purine-thiamine metabolic network of Salmonella

Jannell V. Bazurto, Diana M. Downs

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In Salmonella enterica, 5-aminoimidazole ribonucleotide (AIR) is the precursor of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) pyrophosphate moiety of thiamine and the last intermediate in the common HMP/purine biosynthetic pathway. AIR is synthesized de novo via five reactions catalyzed by the purF, -D, -T, -G, and -I gene products. In vivo genetic analysis demonstrated that in the absence of these gene products AIR can be generated if (i) methionine and lysine are in the growth medium, (ii) PurC is functional, and (iii) 5-amino-4-imidazolecarboxamide ribotide (AICAR) has accumulated. This study provides evidence that the five steps of the common HMP/purine biosynthetic pathway can be bypassed in the synthesis of AIR and thus demonstrates that thiamine synthesis can be uncoupled from the early purine biosynthetic pathway in bacteria.

Original languageEnglish (US)
Pages (from-to)623-631
Number of pages9
JournalGenetics
Volume187
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

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