Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment

Antoine Lesuffleur; Kwan Seop Lim; Nathan C. Lindquist; Hyungsoon Im; Arthur E. Warrington; Moses Rodriguez; Sang Hyun Oh

doi:10.1109/IEMBS.2009.5332429

Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment

Antoine Lesuffleur, Kwan Seop Lim, Nathan C. Lindquist, Hyungsoon Im, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Electrical and Computer Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

9 Scopus citations

Abstract

We integrate periodic nanohole arrays in a thin gold film with lipid membranes in a microfluidic channel. Surface plasmon-enhanced light transmission through the periodic nanohole arrays enables real-time label-free sensing of molecular binding on the lipid membrane surface. This membrane biosensor can potentially act as a natural platform for studying binding kinetics of proteins with their binding partners anchored in the lipid membrane. We also present the concept of using nanopore arrays for kinetic assays of transmembrane proteins in a free-standing lipid membrane.

Original language	English (US)
Title of host publication	Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Subtitle of host publication	Engineering the Future of Biomedicine, EMBC 2009
Publisher	IEEE Computer Society
Pages	1481-1484
Number of pages	4
ISBN (Print)	9781424432967
DOIs	https://doi.org/10.1109/IEMBS.2009.5332429
State	Published - 2009
Event	31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 - Minneapolis, MN, United States Duration: Sep 2 2009 → Sep 6 2009

Publication series

Name	Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009

Other

Other	31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Country/Territory	United States
City	Minneapolis, MN
Period	9/2/09 → 9/6/09

PubMed: MeSH publication types

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10.1109/IEMBS.2009.5332429

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Lesuffleur, A., Lim, K. S., Lindquist, N. C., Im, H., Warrington, A. E., Rodriguez, M., & Oh, S. H. (2009). Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009 (pp. 1481-1484). Article 5332429 (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009). IEEE Computer Society. https://doi.org/10.1109/IEMBS.2009.5332429

Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. / Lesuffleur, Antoine; Lim, Kwan Seop; Lindquist, Nathan C. et al.
Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society, 2009. p. 1481-1484 5332429 (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lesuffleur, A, Lim, KS, Lindquist, NC, Im, H, Warrington, AE, Rodriguez, M & Oh, SH 2009, Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. in Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009., 5332429, Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, IEEE Computer Society, pp. 1481-1484, 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, Minneapolis, MN, United States, 9/2/09. https://doi.org/10.1109/IEMBS.2009.5332429

Lesuffleur A, Lim KS, Lindquist NC, Im H, Warrington AE, Rodriguez M et al. Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society. 2009. p. 1481-1484. 5332429. (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009). doi: 10.1109/IEMBS.2009.5332429

Lesuffleur, Antoine ; Lim, Kwan Seop ; Lindquist, Nathan C. et al. / Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society, 2009. pp. 1481-1484 (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009).

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Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment

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