Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1

Meghdad Hajimorad; Jeffrey A. Gralnick

doi:10.1109/EMBC44109.2020.9176029

Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1

Meghdad Hajimorad, Jeffrey A. Gralnick

Biotechnology Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

To advance synthetic biology approaches that utilize S. oneidensis as host for biotechnology applications, we have investigated the variation in plasmid copy number of a modular vector set resulting from distinct origins of replication under different conditions. The replicons yielded a ≈9X-fold range for plasmid copy number variation in S. oneidensis (while the same origins yielded a ≈3X-fold range in Escherichia coli). This provides a sizeable range to control gene expression levels in S. oneidensis for synthetic biology applications. In addition, plasmid harboring the pBBR1 origin resulted in stable copy numbers in S. oneidensis under different conditions (mid-logarithmic, stationary, multi-plasmid). This may enable the realization of synthetic circuits in S. oneidensis where predictable, quantitative behavior is desired (in either single- or double-plasmid contexts).

Original language	English (US)
Title of host publication	42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society
Subtitle of host publication	Enabling Innovative Technologies for Global Healthcare, EMBC 2020
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	2372-2375
Number of pages	4
ISBN (Electronic)	9781728119908
DOIs	https://doi.org/10.1109/EMBC44109.2020.9176029
State	Published - Jul 2020
Event	42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020 - Montreal, Canada Duration: Jul 20 2020 → Jul 24 2020

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume	2020-July
ISSN (Print)	1557-170X

Conference

Conference	42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020
Country	Canada
City	Montreal
Period	7/20/20 → 7/24/20

PubMed: MeSH publication types

Journal Article

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10.1109/EMBC44109.2020.9176029

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Hajimorad, M., & Gralnick, J. A. (2020). Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1. In 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society: Enabling Innovative Technologies for Global Healthcare, EMBC 2020 (pp. 2372-2375). [9176029] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2020-July). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC44109.2020.9176029

Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1. / Hajimorad, Meghdad; Gralnick, Jeffrey A.

42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society: Enabling Innovative Technologies for Global Healthcare, EMBC 2020. Institute of Electrical and Electronics Engineers Inc., 2020. p. 2372-2375 9176029 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2020-July).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hajimorad, M & Gralnick, JA 2020, Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1. in 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society: Enabling Innovative Technologies for Global Healthcare, EMBC 2020., 9176029, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, vol. 2020-July, Institute of Electrical and Electronics Engineers Inc., pp. 2372-2375, 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020, Montreal, Canada, 7/20/20. https://doi.org/10.1109/EMBC44109.2020.9176029

Hajimorad M, Gralnick JA. Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1. In 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society: Enabling Innovative Technologies for Global Healthcare, EMBC 2020. Institute of Electrical and Electronics Engineers Inc. 2020. p. 2372-2375. 9176029. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC44109.2020.9176029

Hajimorad, Meghdad ; Gralnick, Jeffrey A. / Plasmid copy number variation of a modular vector set in Shewanella oneidensis MR-1. 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society: Enabling Innovative Technologies for Global Healthcare, EMBC 2020. Institute of Electrical and Electronics Engineers Inc., 2020. pp. 2372-2375 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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abstract = "To advance synthetic biology approaches that utilize S. oneidensis as host for biotechnology applications, we have investigated the variation in plasmid copy number of a modular vector set resulting from distinct origins of replication under different conditions. The replicons yielded a ≈9X-fold range for plasmid copy number variation in S. oneidensis (while the same origins yielded a ≈3X-fold range in Escherichia coli). This provides a sizeable range to control gene expression levels in S. oneidensis for synthetic biology applications. In addition, plasmid harboring the pBBR1 origin resulted in stable copy numbers in S. oneidensis under different conditions (mid-logarithmic, stationary, multi-plasmid). This may enable the realization of synthetic circuits in S. oneidensis where predictable, quantitative behavior is desired (in either single- or double-plasmid contexts).",

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