Plasmid colIb contains an ssi signal close to the replication origin

Katsunori Tanaka, Tetsuya Sakai, Yoichi Honda, Hiroshi Hiasa, Hiroshi Sakai, Tohru Komano

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid pSM32, a mini-ColIb plasmid. This ssi signal was situated in the 350-nt HaeIII segment of the 1.8-kb S7 fragment, and located nearly 400 nt downstream of the origin of DNA replication. Introduction of the ssi signal into a mutant of filamentous phage M13 lacking oric resulted in restoration of phage growth and RFI DNA synthesis. Interestingly, DNA homology studies showed that the nucleotide sequence of the ssi signal was extremely homologous with that of the "G4-type" ssi signal in plasmid R100.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
Issue number2
StatePublished - Mar 1991


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