Plasma levels of LH and prolactin were determined in pinealectomized (PX) and/or bilateral ocular enucleated (EX) adult turkey hens maintained in either constant (24LL) or diurnal (16L:8D) lighting. Blood samples were obtained at the following times over a 24-hr period: onset, middle, and end of light period, middle and end of dark period. In experiment 1, the effects of PX alone were evaluated in photorefractory hens exposed to two different photoperiods (24LL and 16L:8D). In experiment 2, the effects of PX and EX were determined in egg-laying and photorefractory hens exposed to a 16L:8D daily photoperiod. Neither PX nor EX significantly altered LH or prolactin levels of egg laying or photorefractory hens at any of the sampling times of the day in either experiment. In experiment 1, LH levels were very similar in both 16L:8D and 24LL but prolactin levels were significantly lower in 24LL than 16L:8D. It was concluded that the pineal gland does not play an essential role in maintaining circulating LH and prolactin levels in constant light or a photoperiod of 16L:8D.