Plasma membrane architecture during hypoxic injury in rat hepatocytes measured by fluorescence quenching and resonance energy transfer imaging

Xue Feng Wang, John J. Lemasters, Brian Herman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Alterations in plasma membrane structure and function are considered of primary importance in the pathogenesis of cell injury. Here, fluorescence quenching imaging and Fluorescence Resonance Energy Transfer (FRET) imaging were used to detail alterations of plasma membrane structure during hypoxic injury in rat hepatocytes. Each of these methods provides complementary information and indicated that hypoxic injury leads to a spatially and temporally heterogeneous increase in the rigidity of the plasma membrane. We propose a model, based on our results, for the explanation of alterations in plasma membrane architecture which occur during hypoxic injury. This model also suggests points at which interventional strategies may be applied to prevent or decrease the extent of the hypoxia‐induced changes in membrane structure and thus prolong or even rescue cells from hypoxic injury.

Original languageEnglish (US)
Pages (from-to)30-39
Number of pages10
JournalBioimaging
Volume1
Issue number1
DOIs
StatePublished - Mar 1993

Keywords

  • Fluorescence Resonance Energy Transfer (FRET) imaging
  • Fluorescence quenching imaging
  • Hypoxic injury
  • Plasma membrane lipid

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