Plasma hormones and expression of growth hormone receptor and insulin-like growth factor-I mRNA in hepatic tissue of periparturient dairy cows

R. P. Radcliff, B. L. McCormack, Brian A Crooker, M. C. Lucy

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Growth hormone plays a central role in the change in nutrient metabolism that occurs during the initiation of lactation. The actions of growth hormone are mediated by the growth hormone receptor (GHR) whose mRNA is present in three alternatively spliced forms (GHR 1A, 1B, and 1C). Liver-specific GHR 1A mRNA is transiently decreased around parturition, but the exact timing of the decline is not known. Our objective was to generate a daily profile for total GHR (GHRtot; all GHR transcripts), GHR 1A, and IGF-I mRNA expression in liver of periparturient Holstein cows and evaluate these daily mRNA profiles relative to daily profiles for periparturient hormones and metabolites. Liver biopsies and blood samples (n = 139) were collected from 65 Holstein cows at the University of Missouri Dairy Farm. At least two cows were sampled on each day from 14 d before to 14 d after parturition. Total cellular RNA was isolated and reverse transcribed to cDNA. Target cDNA were measured by quantitative real-time polymerase chain reaction. Plasma was assayed for progesterone, estradiol, insulin, growth hormone, IGF-I, glucose, and nonesterified fatty acids. The GHR 1A mRNA declined 2 d before parturition, was lowest 3 to 4 d after parturition, and then increased. The IGF-I mRNA declined 1 d after parturition, was lowest 2 to 5 d after parturition and then increased. Total GHR mRNA was not affected by day. The decrease in GHR 1A mRNA was associated with a decrease in progesterone and an increase in estradiol shortly before parturition. A detailed profile of GHR 1A, IGF-I, and GHRtot mRNA expression during the periparturient period was provided. The decreases in GHR 1A and IGF-I during the transition period occurred immediately before (GHR 1A) or shortly after (IGF-I) parturition. Rapid changes in placental and ovarian steroids before parturition were coincident with changes in GHR 1A mRNA.

Original languageEnglish (US)
Pages (from-to)3920-3926
Number of pages7
JournalJournal of Dairy Science
Volume86
Issue number12
DOIs
StatePublished - Jan 1 2003

Fingerprint

Somatotropin Receptors
insulin-like growth factor I
Insulin-Like Growth Factor I
dairy cows
hormones
Hormones
Messenger RNA
liver
Liver
Parturition
parturition
somatotropin
Growth Hormone
cows
somatotropin receptors
tissues
Progesterone
estradiol
Estradiol
progesterone

Keywords

  • Dairy cattle
  • Growth hormone receptor
  • Liver

Cite this

Plasma hormones and expression of growth hormone receptor and insulin-like growth factor-I mRNA in hepatic tissue of periparturient dairy cows. / Radcliff, R. P.; McCormack, B. L.; Crooker, Brian A; Lucy, M. C.

In: Journal of Dairy Science, Vol. 86, No. 12, 01.01.2003, p. 3920-3926.

Research output: Contribution to journalArticle

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