Plasma hippurate in renal failure: High-performance liquid chromatography method and clinical application

P. Igarashi, P. Gulyassy, L. Stanfel, T. Depner

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have developed a high-performance liquid chromatography (HPLC) method for assay of hippurate in plasma of patients with renal failure. Hippurate accounts, in part, for the impaired binding of drugs and metabolites to albumin and may cause other disorders in azotemic patients. The method is precise, accurate and reproducible. Among 25 patients with acute and chronic renal failure having serum creatinine in the range of 2.9-43 mg/dl (256-3,801 μmol/l), plasma hippurate ranged from 0.11 to 16.2 mg/dl (6.1-904 μmol/l). Hippurate concentration correlated moderately closely with plasma creatinine, urea and anion gap. Its curvilinear relation to the reciprocal of serum creatinine indicated a proportional decline of GFR and tubular function or the accumulation of inhibitors of the proximal tubular anion secretory pathway. The method should be useful for further studies of abnormal albumin binding as well as other disorders in azotemic patients.

Original languageEnglish (US)
Pages (from-to)290-294
Number of pages5
JournalNephron
Volume47
Issue number4
DOIs
StatePublished - 1987

Bibliographical note

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Fingerprint Dive into the research topics of 'Plasma hippurate in renal failure: High-performance liquid chromatography method and clinical application'. Together they form a unique fingerprint.

Cite this