Plant Shoot Tip Response to Treatment with Plant Vitrification Solution # 2

G. M. Volk, A. D. Henk, R. M. Bonnart, A. Shepherd, B. L. Gross

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations


Plant cryopreservation has become an effective method to conserve desirable genotypes of vegetatively propagated plant species. In many cases, excised shoot tips are treated with cryoprotectant solutions to prevent the formation of lethal ice during the freezing process. Plant vitrification solution 2 (PVS2) is a highly effective cryoprotectant solution that desiccates, permeates, and changes the freezing properties within shoot tips. We summarize a series of experiments that have been performed to better understand the mechanism by which PVS2 interacts with plant cells. Our program has measured toxicity, calorimetric, structural, and genetic responses to PVS2 and LN treatments within the shoot tips of model systems. Despite the toxicity of PVS2, it remains a highly effective vitrification solution for plant shoot tips systems.

Original languageEnglish (US)
Title of host publicationII International Symposium on Plant Cryopreservation
PublisherInternational Society for Horticultural Science
Number of pages4
ISBN (Print)9789462610279
StatePublished - Jun 22 2014

Publication series

NameActa Horticulturae
ISSN (Print)0567-7572


  • Cryopreservation
  • Gene expression
  • Liquid nitrogen


Dive into the research topics of 'Plant Shoot Tip Response to Treatment with Plant Vitrification Solution # 2'. Together they form a unique fingerprint.

Cite this