Plant genome editing and the relevance of off-target changes1[OPEN]

Nathaniel Graham, Gunvant B. Patil, David M. Bubeck, Raymond C. Dobert, Kevin C. Glenn, Annie T. Gutsche, Sandeep Kumar, John A. Lindbo, Luis Maas, Gregory D. May, Miguel E. Vega-Sanchez, Robert M. Stupar, Peter L. Morrell

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to introduce precise genetic changes into plants. Like traditional approaches, such as conventional crossing and induced mutagenesis, genome editing aims to improve crop yield and nutrition. Next-generation sequencing studies demonstrate that across their genomes, populations of crop species typically carry millions of single nucleotide polymorphisms and many copy number and structural variants. Spontaneous mutations occur at rates of;1028 to 1029 per site per generation, while variation induced by chemical treatment or ionizing radiation results in higher mutation rates. In the context of SDNs, an off-target change or edit is an unintended, nonspecific mutation occurring at a site with sequence similarity to the targeted edit region. SDN-mediated off-target changes can contribute to a small number of additional genetic variants compared to those that occur naturally in breeding populations or are introduced by induced-mutagenesis methods. Recent studies show that using computational algorithms to design genome editing reagents can mitigate off-target edits in plants. Finally, crops are subject to strong selection to eliminate off-type plants through well-established multigenerational breeding, selection, and commercial variety development practices. Within this context, off-target edits in crops present no new safety concerns compared to other breeding practices. The current generation of genome editing technologies is already proving useful to develop new plant varieties with consumer and farmer benefits. Genome editing will likely undergo improved editing specificity along with new developments in SDN delivery and increasing genomic characterization, further improving reagent design and application.

Original languageEnglish (US)
Pages (from-to)1453-1471
Number of pages19
JournalPlant physiology
Volume183
Issue number4
DOIs
StatePublished - Aug 2020
Externally publishedYes

Bibliographical note

Funding Information:
1This work was supported by the U.S. Department of Agriculture/ National Institute of Food and Agriculture (grant nos. 2015–33522–24096 and 2019–33522–30200 to R.M.S. and P.L.M.). 2Present address: Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409. 3Author for contact: pmorrell@umn.edu. 4Senior author. All the authors conceived, drafted, edited, and critically reviewed the text, tables, and figures of this article. [OPEN]Articles can be viewed without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.19.01194

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

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