Planar chromatography and immunodetection of hydrocarbons on polyvinylidene difluoride membranes

Fernando Medina Ferrer, Jake V. Bailey

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Fast, cheap, and simple separation of lipids and hydrocarbons can currently be achieved using thin-layer chromatography. Here, we describe an alternative planar chromatographic method using polyvinylidene difluoride membranes as the stationary phase. The procedure has the same advantages of thin-layer chromatography over other expensive and time-consuming techniques, such as high-performance liquid chromatography or gas chromatography. Polyvinylidene difluoride membranes, however, also provide an immediate support for analyte development via immunodetection, are easy to manipulate, and potentially increase the performance of other detection methods. We show that polyvinylidene difluoride membranes are compatible with a variety of solvents that can migrate by capillarity and redistribute analytes between the membrane and the solvent according to their relative affinities, providing a chromatographic separation. We directly test the developed membranes by immunoblotting using anti-squalene antibodies that cross-react with acyclic isoprenoids. Separations of crude oils and plant extracts under different solvent conditions show the potential to resolve hydrocarbon group types and also to provide characteristic fingerprints of plant pigments and squalene degradation products. Polyvinylidene difluoride membranes prove useful as a stationary phase for planar chromatography and for the subsequent immunodetection of the separated compounds, providing a new and simple chromatographic technique to analyze lipids and hydrocarbons.

Original languageEnglish (US)
Pages (from-to)3654-3664
Number of pages11
JournalJournal of Separation Science
Volume44
Issue number19
Early online dateJul 29 2021
DOIs
StatePublished - Sep 2021

Bibliographical note

Funding Information:
We thank Alan L. Epstein of the Keck School of Medicine at the University of Southern California for antibody production. We are grateful for the anonymous reviewers and editor that helped improve the quality of this manuscript. This work was supported by a doctoral fellowship to FMF by Fulbright #15150776, by CONICYT folio-72160214, and by the UMN Graduate School DDF. Acknowledgment is made to the donors of the American Chemical Society Petroleum Research Fund for support of this research via a New Directions award to J.V.B. (PRF#56972-ND2).

Funding Information:
We thank Alan L. Epstein of the Keck School of Medicine at the University of Southern California for antibody production. We are grateful for the anonymous reviewers and editor that helped improve the quality of this manuscript. This work was supported by a doctoral fellowship to FMF by Fulbright #15150776, by CONICYT folio‐72160214, and by the UMN Graduate School DDF. Acknowledgment is made to the donors of the American Chemical Society Petroleum Research Fund for support of this research via a New Directions award to J.V.B. (PRF#56972‐ND2).

Publisher Copyright:
© 2021 Wiley-VCH GmbH

Keywords

  • far-eastern blot
  • immunoblotting
  • phytol
  • squalene
  • thin layer chromatography

PubMed: MeSH publication types

  • Journal Article

Fingerprint

Dive into the research topics of 'Planar chromatography and immunodetection of hydrocarbons on polyvinylidene difluoride membranes'. Together they form a unique fingerprint.

Cite this