Abstract
Maternal pre-pregnancy obesity may have an impact on both maternal and fetal health. We examined the microbiome recovered from placentas in a multi-ethnic maternal pre-pregnant obesity cohort, through an optimized microbiome protocol to enrich low bacterial biomass samples. We found that the microbiomes recovered from the placentas of obese pre-pregnant mothers are less abundant and less diverse when compared to those from mothers of normal pre-pregnancy weight. Microbiome richness also decreases from the maternal side to the fetal side, demonstrating heterogeneity by geolocation within the placenta. In summary, our study shows that the microbiomes recovered from the placentas are associated with pre-pregnancy obesity. Importance: Maternal pre-pregnancy obesity may have an impact on both maternal and fetal health. The placenta is an important organ at the interface of the mother and fetus, and supplies nutrients to the fetus. We report that the microbiomes enriched from the placentas of obese pre-pregnant mothers are less abundant and less diverse when compared to those from mothers of normal pre-pregnancy weight. More over, the microbiomes also vary by geolocation within the placenta.
Original language | English (US) |
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Article number | e21524 |
Journal | FASEB Journal |
Volume | 35 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2021 |
Bibliographical note
Funding Information:We would like to thank Dr. Vincent Young for giving helpful suggestions in preparing this manuscript. Dr. Lana X Garmire?s research is supported by grants K01ES025434 awarded by NIEHS through funds provided by the trans-NIH Big Data to Knowledge (BD2K) initiative (http://datascience.nih.gov/bd2k), P20 COBRE GM103457 awarded by NIH/NIGMS, R01 LM012373 awarded by NLM, and R01 HD084633 awarded by NICHD to LX Garmire. Funding was also provided in part by the Department of Obstetrics and Gynecology, University of Hawaii. 16S sequencing was performed by The University of Minnesota Genomics Center.
Publisher Copyright:
© 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
Keywords
- 16S sequencing
- Microbiome
- Obesity
- Placenta
- Pregnancy