TY - JOUR
T1 - PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression
AU - Lim, Tae Gyu
AU - Kim, Yoon A.
AU - Kim, Jong Eun
AU - Baek, Sohee
AU - Lee, Sung Young
AU - Lee, Charles C.
AU - Chen, Hanyong
AU - Kim, Jong Rhan
AU - Kwon, Jung Yeon
AU - Bode, Ann M.
AU - Lee, Chang Yong
AU - Dong, Zigang
AU - Oh, Deok Kun
AU - Park, Jun Seong
AU - Lee, Ki Won
N1 - Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2018/5
Y1 - 2018/5
N2 - The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.
AB - The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.
KW - 3D culture
KW - 7,8,4′-trihydroxyisoflavone
KW - Protein kinase C iota
KW - matrix metalloproteinase-1
UR - http://www.scopus.com/inward/record.url?scp=85022321687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85022321687&partnerID=8YFLogxK
U2 - 10.1111/exd.13375
DO - 10.1111/exd.13375
M3 - Article
C2 - 28453925
AN - SCOPUS:85022321687
SN - 0906-6705
VL - 27
SP - 449
EP - 452
JO - Experimental Dermatology
JF - Experimental Dermatology
IS - 5
ER -
