Pineal modulation of ACTH 1-17 effect upon murine corticosterone production

Salvador Sáchez De La Pena, Franz Halberg, Erna Halberg, Frank Ungar, Germaine Cornelissen, Elizabeth Sánchez, Gregory Brown, Lawrence E. Scheving, Edmond G. Yunis, Paul Vecsei

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30 Scopus citations


In tests of Corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37°C with 95% O2 and 5% CO2, the media were stored at -20°C until Corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal Corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalBrain Research Bulletin
Issue number1
StatePublished - Jul 1983


  • ACTH 1-17
  • Adrenal
  • Amplification
  • Attenuation
  • Feed-sideward
  • Incubation
  • Pineal


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