TY - JOUR
T1 - Pineal modulation of ACTH 1-17 effect upon murine corticosterone production
AU - De La Pena, Salvador Sáchez
AU - Halberg, Franz
AU - Halberg, Erna
AU - Ungar, Frank
AU - Cornelissen, Germaine
AU - Sánchez, Elizabeth
AU - Brown, Gregory
AU - Scheving, Lawrence E.
AU - Yunis, Edmond G.
AU - Vecsei, Paul
PY - 1983/7
Y1 - 1983/7
N2 - In tests of Corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37°C with 95% O2 and 5% CO2, the media were stored at -20°C until Corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal Corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.
AB - In tests of Corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37°C with 95% O2 and 5% CO2, the media were stored at -20°C until Corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal Corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.
KW - ACTH 1-17
KW - Adrenal
KW - Amplification
KW - Attenuation
KW - Feed-sideward
KW - Incubation
KW - Pineal
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U2 - 10.1016/0361-9230(83)90060-6
DO - 10.1016/0361-9230(83)90060-6
M3 - Article
C2 - 6313140
AN - SCOPUS:0020559563
SN - 0361-9230
VL - 11
SP - 117
EP - 125
JO - Brain Research Bulletin
JF - Brain Research Bulletin
IS - 1
ER -