Abstract
We report a method for the analysis of picomolar concentration proteins using electrophoretically mediated microanalysis (EMMA) to label proteins on-column with a fluorogenic reagent. Labeling is followed by capillary zone electrophoresis separation and postcolumn detection based on laser-induced fluorescence. The method provides a concentration detection limit (3σ) of 3 × 10-13 M for conalbumin. The method provides separation efficiency of 300 000 theoretical plates. Protein extract from a human colon adenocarcinoma cell line generated a dozen major components and many minor components in a 12-min separation; the protein extract from 2.5 cells was used for this analysis. When compared to UV absorbance detection, the EMMA method provides 7 000 000-fold improvement in detection limit.
Original language | English (US) |
---|---|
Pages (from-to) | 4546-4548 |
Number of pages | 3 |
Journal | Analytical chemistry |
Volume | 70 |
Issue number | 21 |
DOIs | |
State | Published - Nov 1 1998 |