Physiological responses of emerald ash borer larvae to feeding on different ash species reveal putative resistance mechanisms and insect counter-adaptations

C. M. Rigsby, D. N. Showalter, D. A. Herms, J. L. Koch, P. Bonello, D. Cipollini

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Emerald ash borer, Agrilus planipennis Fairmaire, an Asian wood-boring beetle, has devastated ash (. Fraxinus spp.) trees in North American forests and landscapes since its discovery there in 2002. In this study, we collected living larvae from EAB-resistant Manchurian ash (. Fraxinus mandschurica), and susceptible white (. Fraxinus americana) and green (. Fraxinus pennsylvanica) ash hosts, and quantified the activity and production of selected detoxification, digestive, and antioxidant enzymes. We hypothesized that differences in larval physiology could be used to infer resistance mechanisms of ash. We found no differences in cytochrome P450, glutathione-S-transferase, carboxylesterase, sulfotransferase, and tryptic BA. pNAase activities between larvae feeding on different hosts. Despite this, Manchurian ash-fed larvae produced a single isozyme of low electrophoretic mobility that was not produced in white or green ash-fed larvae. Additionally, larvae feeding on white and green ash produced two serine protease isozymes of high electrophoretic mobility that were not observed in Manchurian ash-fed larvae. We also found lower activity of β-glucosidase and higher activities of monoamine oxidase, ortho-quinone reductase, catalase, superoxide dismutase, and glutathione reductase in Manchurian ash-fed larvae compared to larvae that had fed on susceptible ash. A single isozyme was detected for both catalase and superoxide dismutase in all larval groups. The activities of the quinone-protective and antioxidant enzymes are consistent with the resistance phenotype of the host species, with the highest activities measured in larvae feeding on resistant Manchurian ash. We conclude that larvae feeding on Manchurian ash could be under quinone and oxidative stress, suggesting these may be potential mechanisms of resistance of Manchurian ash to EAB larvae, and that quinone-protective and antioxidant enzymes are important counter-adaptations of larvae for dealing with these resistance mechanisms.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalJournal of Insect Physiology
Volume78
DOIs
StatePublished - Jul 1 2015

Bibliographical note

Funding Information:
We thank Dr. Michael Leffak and Joanna Bartholomew (Wright State University) for use of their gel imager. Jonathan Lelito (USDA-APHIS) and Therese Poland (Michigan State University) provided EAB eggs. Robert Hansen, Alejandro Chiriboga, Amilcar Vargas, Diane Hartzler, Paul Snyder, Amy Hill, Anna Conrad, Patrick Sherwood, Caterina Villari, Mark Miller, Mary Mason (all from The Ohio State University), and David Carey (USDA Forest Service Northern Research Station) assisted with maintaining ash trees and maintaining and collecting larvae. This project was funded by the USDA APHIS Accelerated Emerald Ash Borer Research Program, by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, and Wright State University.

Publisher Copyright:
© 2015 Elsevier Ltd.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Adaptations to host defense
  • Antioxidant enzymes
  • Detoxification
  • Host plant resistance
  • Oxidative stress
  • Wood-boring insects

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