Physical mapping of the Saccharomyces cerevisiae Ap4A phosphorylase I-encoding gene by the Achilles' cleavage method

Preston N. Garrison, Michael Koob, Larry D. Barnes

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Abstract

LacI-mediated Achilles' cleavage (AC) is a method for selective fragmentation of chromosomes at special lac operator sites introduced by gene targeting methods [Koob and Szybalski, Science 250 (1990) 271-273]. The Saccharomyces cerevisiae APA1 gene, coding for diadenosine 5′, 5‴-P1, P4-tetraphosphate phosphorylase I, has previously been shown to be located on chromosome III [Kaushal et al., Gene 95 (1990) 79-84]. We have now used the AC method to map APA1 gene to a site 44 kb from the left terminus of the chromosome, between the HIS4 and HML genes. This location was confirmed by the comparison of restriction maps of the APA1 gene region to published restriction maps of chromosome III.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalGene
Volume111
Issue number1
DOIs
StatePublished - Feb 1 1992

Bibliographical note

Funding Information:
We thank Carol Newlon for a plasmid and Ray McKee for sharing sequence data before publication. The sequencing of B9G by McKee et al. was part of the project to sequence chromosome III of the Biotechnology Action Programme of the Commission of the European Communities. This work was supported by grant GM-27220 (to L.D.B.) from the National Institute of General Medical Sciences. The AC procedures, as carried out by M.K. in the McArdle Laboratory, University of Wisconsin, were supported by the NIH grant No. 1RO1HG00379-01 from the National Center for Human Genome Research to Dr. W. Szybalski, who initiated the present collaborative effort.

Keywords

  • APA1 gene
  • Recombinant DNA
  • chromosome III
  • chromosome fragmentation
  • diadenosine 5′, 5‴-P, P-tetraphosphate
  • yeast

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