LacI-mediated Achilles' cleavage (AC) is a method for selective fragmentation of chromosomes at special lac operator sites introduced by gene targeting methods [Koob and Szybalski, Science 250 (1990) 271-273]. The Saccharomyces cerevisiae APA1 gene, coding for diadenosine 5′, 5‴-P1, P4-tetraphosphate phosphorylase I, has previously been shown to be located on chromosome III [Kaushal et al., Gene 95 (1990) 79-84]. We have now used the AC method to map APA1 gene to a site 44 kb from the left terminus of the chromosome, between the HIS4 and HML genes. This location was confirmed by the comparison of restriction maps of the APA1 gene region to published restriction maps of chromosome III.
Bibliographical noteFunding Information:
We thank Carol Newlon for a plasmid and Ray McKee for sharing sequence data before publication. The sequencing of B9G by McKee et al. was part of the project to sequence chromosome III of the Biotechnology Action Programme of the Commission of the European Communities. This work was supported by grant GM-27220 (to L.D.B.) from the National Institute of General Medical Sciences. The AC procedures, as carried out by M.K. in the McArdle Laboratory, University of Wisconsin, were supported by the NIH grant No. 1RO1HG00379-01 from the National Center for Human Genome Research to Dr. W. Szybalski, who initiated the present collaborative effort.
- APA1 gene
- Recombinant DNA
- chromosome III
- chromosome fragmentation
- diadenosine 5′, 5‴-P, P-tetraphosphate