Phylogeography, salinity adaptations and metabolic potential of the candidate division KB1 bacteria based on a partial single cell genome

Lisa M. Nigro, Andrew S. Hyde, Barbara J. MacGregor, Andreas Teske

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Abstract

Deep-sea hypersaline anoxic basins and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that have been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis - previously developed based on 14C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source.

Original languageEnglish (US)
Article number1266
JournalFrontiers in Microbiology
Volume7
Issue numberAUG
DOIs
StatePublished - Aug 22 2016
Externally publishedYes

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Keywords

  • Candidate Division KB1
  • Genome
  • Hypersaline
  • Orca Basin
  • Phylogeography

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