Phylogenomic Analysis of Extraintestinal Pathogenic Escherichia coli Sequence Type 1193, an Emerging Multidrug-Resistant Clonal Group

Tim Johnson, Ehud Elnekave, Elizabeth A. Miller, Jeannette Munoz-Aguayo, Cristian Flores Figueroa, Brian D Johnston, Daniel W. Nielson, Catherine M. Logue, James R Johnson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The fluoroquinolone-resistant sequence type 1193 (ST1193) of Escherichia coli, from the ST14 clonal complex (STc14) within phylogenetic group B2, has appeared recently as an important cause of extraintestinal disease in humans. Although this emerging lineage has been characterized to some extent using conventional methods, it has not been studied extensively at the genomic level. Here, we used whole-genome sequence analysis to compare 355 ST1193 isolates with 72 isolates from other STs within STc14. Using core genome phylogeny, the ST1193 isolates formed a tightly clustered clade with many genotypic similarities, unlike ST14 isolates. All ST1193 isolates possessed the same set of three chromosomal mutations conferring fluoroquinolone resistance, carried the fimH64 allele, and were lactose non-fermenting. Analysis revealed an evolutionary progression from K1 to K5 capsular types and acquisition of an F-type virulence plasmid, followed by changes in plasmid structure congruent with genome phylogeny. In contrast, the numerous identified antimicrobial resistance genes were distributed incongruently with the underlying phylogeny, suggesting frequent gain or loss of the corresponding resistance gene cassettes despite retention of the presumed carrier plasmids. Pangenome analysis revealed gains and losses of genetic loci occurring during the transition from ST14 to ST1193 and from the K1 to K5 capsular types. Using time-scaled phylogenetic analysis, we estimated that current ST1193 clades first emerged approximately 25 years ago. Overall, ST1193 appears to be a recently emerged clone in which both stepwise and mosaic evolution have contributed to epidemiologic success.

Original languageEnglish (US)
Article numbere01913-18
JournalAntimicrobial agents and chemotherapy
Volume63
Issue number1
DOIs
StatePublished - Jan 1 2019

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Phylogeny
Fluoroquinolones
Genome
Plasmids
F Factor
Genetic Loci
Lactose
Genes
Sequence Analysis
Virulence
Clone Cells
Alleles
Escherichia coli
Mutation
Extraintestinal Pathogenic Escherichia coli

Keywords

  • Antimicrobial resistance
  • ExPEC
  • Fluoroquinolone
  • Plasmid
  • ST1193

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Phylogenomic Analysis of Extraintestinal Pathogenic Escherichia coli Sequence Type 1193, an Emerging Multidrug-Resistant Clonal Group. / Johnson, Tim; Elnekave, Ehud; Miller, Elizabeth A.; Munoz-Aguayo, Jeannette; Figueroa, Cristian Flores; Johnston, Brian D; Nielson, Daniel W.; Logue, Catherine M.; Johnson, James R.

In: Antimicrobial agents and chemotherapy, Vol. 63, No. 1, e01913-18, 01.01.2019.

Research output: Contribution to journalArticle

Johnson, Tim ; Elnekave, Ehud ; Miller, Elizabeth A. ; Munoz-Aguayo, Jeannette ; Figueroa, Cristian Flores ; Johnston, Brian D ; Nielson, Daniel W. ; Logue, Catherine M. ; Johnson, James R. / Phylogenomic Analysis of Extraintestinal Pathogenic Escherichia coli Sequence Type 1193, an Emerging Multidrug-Resistant Clonal Group. In: Antimicrobial agents and chemotherapy. 2019 ; Vol. 63, No. 1.
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