Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activity

Laurie L. Parker, Shawn B. Brueggemeier, Won Jun Rhee, Ding Wu, Stephen B.H. Kent, Stephen J. Kron, Sean P. Palecek

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We have developed an acrylamide copolymerization strategy to immobilize acrylamide labeled peptides and proteins into a hydrogel surface and detect their modifications using MALDI-TOF mass spectrometry. Copolymerization into hydrogels is robust, compatible with "off-the-shelf" chemistry, and yields materials and surfaces that are stable to aqueous or organic solvents, drying, high or low temperature, high or low pH, oxidizing agents, sonication, mechanical contact, etc. The use of acrylamide hydrogels allows immobilization of substrates in a hydrated environment that can be used both as a biological reaction matrix and as a MALDI target. In our strategy, a substrate peptide was designed in a modular fashion to include both modification site and affinity domains. It was labeled with an acrylamide functionality using a generalized chemistry and covalently attached to the surface with a photocleavable linker, allowing for aggressive washing to remove any fouling, followed by selective release for MALDI-TOF analysis. Using this system we were able to analyze and compare v-Abl (truncated) and c-Abl (full-length) kinase activity on a peptide substrate with an affinity domain specific for the full-length kinase, observing excellent overall reproducibility in the extent of phosphorylation detected. This work serves as proof of principle for modular substrate design strategies for mass spectrometry-readable biosensors.

Original languageEnglish (US)
Pages (from-to)1097-1104
Number of pages8
JournalAnalyst
Volume131
Issue number10
DOIs
StatePublished - 2006

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