Photoactivated localization microscopy for cellular imaging

Paulina Achurra, Seamus Holden, Thomas Pengo, Suliana Manley

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

In a method termed photoactivated localization microscopy (PALM), super-resolution fluorescence imaging can be achieved through the localization of single molecules. This allows the resolution of specific proteins fused to the appropriate fluorescent protein label. Here, we summarize fluorescent proteins suitable for PALM, the technical aspects of multicolor and three-dimensional imaging, and the software packages that are available. Additionally, we highlight several biological applications with an emphasis on neuroscience.

Original languageEnglish (US)
Title of host publicationSuper-Resolution Microscopy Techniques in the Neurosciences
PublisherHumana Press Inc.
Pages87-111
Number of pages25
ISBN (Print)9781627039826
DOIs
StatePublished - 2014

Publication series

NameNeuromethods
Volume86
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Data analysis for PALM
  • Fluorescence proteins
  • Live imaging
  • Super-resolution microscopy

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