Photoactivated localization microscopy for cellular imaging

Paulina Achurra, Seamus Holden, Thomas Pengo, Suliana Manley

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


In a method termed photoactivated localization microscopy (PALM), super-resolution fluorescence imaging can be achieved through the localization of single molecules. This allows the resolution of specific proteins fused to the appropriate fluorescent protein label. Here, we summarize fluorescent proteins suitable for PALM, the technical aspects of multicolor and three-dimensional imaging, and the software packages that are available. Additionally, we highlight several biological applications with an emphasis on neuroscience.

Original languageEnglish (US)
Title of host publicationSuper-Resolution Microscopy Techniques in the Neurosciences
PublisherHumana Press Inc.
Number of pages25
ISBN (Print)9781627039826
StatePublished - 2014

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • Data analysis for PALM
  • Fluorescence proteins
  • Live imaging
  • Super-resolution microscopy


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