Photoacoustic probing of fluorophore excited state lifetime with application to oxygen sensing

Shai Ashkenazi, Shen Wen Huang, Thomas Horvath, Yong Eun L Koo, Raoul Kopelman

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


A new method is developed to perform local measurements of fluorophore excited state lifetimes in turbid media without collecting the fluorescence emission. The method is based on a pump-probe approach where a first laser pulse excites the dye and then a second laser pulse is used for photoacoustic probing of the transient absorption. The photoacoustic response generated by the probe pulse is recorded by an ultrasound receiver. Repeating the measurement for increasing pump-probe time delays yields a series of photoacoustic signals that are used to extract the lifetime of the excited state. The method is validated by measuring the lifetime of an oxygen sensitive dye solution at different concentrations of dissolved oxygen. The dye is pumped with a 532-nm pulsed laser and the transient absorption at 740nm is probed using a second pulsed laser system. The photoacoustic-based results are in close agreement with those obtained from time-dependent fluorescent measurements. The method can be extended to photoacoustic lifetime imaging by using a receiver array instead of a single receiver. Potential applications of this method include tissue oxygen imaging for cancer diagnostics and mapping molecular events such as resonant energy transfer and ion collisions in a biological environment.

Original languageEnglish (US)
Article number034023
JournalJournal of biomedical optics
Issue number3
StatePublished - 2008


  • fluorescence lifetime imaging
  • molecular imaging
  • oxygen sensing
  • photoacoustic imaging
  • transient absorption


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