Photo-CIDNP nuclear magnetic resonance as a probe for conformational changes in epidermal growth factor

K. H. Mayo, A. De Marco, R. Kaptein

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Photo-chemically induced dynamic nuclear polarization (CIDNP)-NMR spectroscopy at 360 MHz has been used to investigate pH-induced conformational transitions in mouse epidermal growth factor. At about pH 9, all five tyrosine residues and both tryptophan residues are, to various extents, solvent-exposed, while the His-22 residue is buried in the protein matrix. Tyr-13 is the least exposed of the tyrosine residues and also the most immobilized. As the pH is decreased to 5.9, the tryptophan residues gradually become less exposed, while the Tyr-13 residue becomes internalized in the protein. These data suggest that the C-terminus and part of the N-terminal structural domain are affected by a conformational transition in mouse epidermal growth factor occurring between pH 6 and 8 via breakage of the His-22 inter-residue linkage. Above pH 9, a decreased photo-CIDNP effect is evident for both tryptophans and for Tyr-10 and Tyr-13; this information suggests that a second conformational change takes place at basic pH, which may simply be incipient denaturation.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume874
Issue number2
DOIs
StatePublished - Nov 21 1986

Keywords

  • (Mouse)
  • CIDNP
  • Conformational change
  • Epidermal growth factor
  • H-NMR spectroscopy
  • two-dimensional NMR

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